Regulation of bacterial RNase P ribozyme reaction by divalent cation and guide DNA.

Tomoaki Ando, Terumichi Tanaka, Yoshiaki Hori, Yo Kikuchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The RNA subunit of bacterial ribonuclease P (RNase P) is a ribozyme which can cleave a canonical cloverleaf tRNA precursor and a hairpin RNA with a CCA-3' tag sequence as its substrate. With high concentration of Mg ion, the ribozyme as well as holo enzyme internally cleaves certain tRNAs in vitro. We denoted this unusual reaction as hyperprocessing. By controlling magnesium ion concentration for the reaction and also by forcing the RNA shape with external guide DNAs, we could regulate the hyperprocessing reaction by the bacterial RNase P enzymes. These techniques will lead the RNase P ribozyme to more designable and more applicable RNA-cleaving enzyme.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages271-272
Number of pages2
Edition2
Publication statusPublished - 2002
Externally publishedYes

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    Ando, T., Tanaka, T., Hori, Y., & Kikuchi, Y. (2002). Regulation of bacterial RNase P ribozyme reaction by divalent cation and guide DNA. In Nucleic acids research. Supplement (2001) (2 ed., pp. 271-272)