Regulation of ribozyme activity by engineered protein switch.

Terumichi Tanaka*, Naomi Kanda, Yo Kikuchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.

Original languageEnglish
Pages (from-to)343-344
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
Publication statusPublished - 2005
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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