Regulation of ribozyme activity by engineered protein switch.

Terumichi Tanaka; Naomi Kanda; Yo Kikuchi

Regulation of ribozyme activity by engineered protein switch.

Terumichi Tanaka, Naomi Kanda, Yo Kikuchi

Research output: Contribution to journal › Article › peer-review

Abstract

Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.

Original language	English
Pages (from-to)	343-344
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	49
Publication status	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Access to Document

Fingerprint Dive into the research topics of 'Regulation of ribozyme activity by engineered protein switch.'. Together they form a unique fingerprint.

Cite this

@article{e2a167f42cbe4a4285d66c8eaec264cb,

title = "Regulation of ribozyme activity by engineered protein switch.",

abstract = "Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.",

author = "Terumichi Tanaka and Naomi Kanda and Yo Kikuchi",

year = "2005",

language = "English",

pages = "343--344",

journal = "Nucleic acids symposium series (2004)",

issn = "1746-8272",

publisher = "Oxford University Press",

number = "49",

}

TY - JOUR

T1 - Regulation of ribozyme activity by engineered protein switch.

AU - Tanaka, Terumichi

AU - Kanda, Naomi

AU - Kikuchi, Yo

PY - 2005

Y1 - 2005

N2 - Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.

AB - Previously, we showed that the P3 domain of the Escherichia coli ribonuclease P ribozyme can be truncated and replaced in vitro. In this study, we prepared a P3-replaced variant of the E. coli ribozyme that has HIV TAR sequence as the engineered P3 domain. The mutant ribozyme demonstrated the ribonuclease P activity and was inhibited in the presence of the HIV tat protein fragment. Our results showed that the P3 domain of this enzyme can be engineered, and addition of some heterologous protein subunits can also be done to this domain.

UR - http://www.scopus.com/inward/record.url?scp=39049176450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049176450&partnerID=8YFLogxK

M3 - Article

C2 - 17150774

AN - SCOPUS:39049176450

SP - 343

EP - 344

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

SN - 1746-8272

IS - 49

ER -