Regulation of serum thrombopoietin levels by platelets and megakaryocytes in patients with aplastic anaemia and idiopathic thrombocytopenic purpura

Naoaki Ichikawa, Fumihiro Ishida, Shigetaka Shimodaira, Tomoyuki Tahara, Takashi Kato, Kiyoshi Kitano

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

To clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 ± 9.71 f mol/ml (mean ± SD) and 1.66 ± 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 ± 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalThrombosis and Haemostasis
Volume76
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Inosine Triphosphate
Thrombopoietin
Idiopathic Thrombocytopenic Purpura
Megakaryocytes
Aplastic Anemia
Blood Platelets
Serum
Platelet Count
Bone Marrow
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Hematology

Cite this

Regulation of serum thrombopoietin levels by platelets and megakaryocytes in patients with aplastic anaemia and idiopathic thrombocytopenic purpura. / Ichikawa, Naoaki; Ishida, Fumihiro; Shimodaira, Shigetaka; Tahara, Tomoyuki; Kato, Takashi; Kitano, Kiyoshi.

In: Thrombosis and Haemostasis, Vol. 76, No. 2, 1996, p. 156-160.

Research output: Contribution to journalArticle

Ichikawa, Naoaki ; Ishida, Fumihiro ; Shimodaira, Shigetaka ; Tahara, Tomoyuki ; Kato, Takashi ; Kitano, Kiyoshi. / Regulation of serum thrombopoietin levels by platelets and megakaryocytes in patients with aplastic anaemia and idiopathic thrombocytopenic purpura. In: Thrombosis and Haemostasis. 1996 ; Vol. 76, No. 2. pp. 156-160.
@article{83b3fd4b0e7c4c0bbe88bc977fb4b87e,
title = "Regulation of serum thrombopoietin levels by platelets and megakaryocytes in patients with aplastic anaemia and idiopathic thrombocytopenic purpura",
abstract = "To clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 ± 9.71 f mol/ml (mean ± SD) and 1.66 ± 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 ± 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.",
author = "Naoaki Ichikawa and Fumihiro Ishida and Shigetaka Shimodaira and Tomoyuki Tahara and Takashi Kato and Kiyoshi Kitano",
year = "1996",
language = "English",
volume = "76",
pages = "156--160",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "2",

}

TY - JOUR

T1 - Regulation of serum thrombopoietin levels by platelets and megakaryocytes in patients with aplastic anaemia and idiopathic thrombocytopenic purpura

AU - Ichikawa, Naoaki

AU - Ishida, Fumihiro

AU - Shimodaira, Shigetaka

AU - Tahara, Tomoyuki

AU - Kato, Takashi

AU - Kitano, Kiyoshi

PY - 1996

Y1 - 1996

N2 - To clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 ± 9.71 f mol/ml (mean ± SD) and 1.66 ± 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 ± 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.

AB - To clarify the regulatory mechanism of thrombopoietin (TPO, c-Mpl ligand) in chronic thrombocytopenic conditions, we determined TPO levels in the sera of patients with aplastic anaemia (AA; n = 26) and idiopathic thrombocytopenic purpura (ITP; n = 32) by an enzyme-linked immunosorbent assay. Despite a similarity in platelet counts, serum TPO levels in the AA group were markedly higher than those in the ITP group: 20.41 ± 9.71 f mol/ml (mean ± SD) and 1.66 ± 0.55 f mol/ml, respectively, both of which were significantly elevated compared to normal subjects (n = 41; 1.22 ± 0.37). In both groups, serum TPO level showed an inverse correlation with the platelet count. We determined the megakaryocyte volume using bone marrow clot section and found that it was markedly small in the AA group; while in the ITP group it was augmented with a correlation to serum TPO level. Our findings suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP.

UR - http://www.scopus.com/inward/record.url?scp=0029817844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029817844&partnerID=8YFLogxK

M3 - Article

C2 - 8865522

AN - SCOPUS:0029817844

VL - 76

SP - 156

EP - 160

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 2

ER -