Regulation of sialyl-Lewis x epitope expression by TNF-α and EGF in an airway carcinoma cell line

Yuji Ishibashi, Yoshio Inouye, Teruo Okano, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Sialyl-Lewis x epitopes and MUC5AC protein are known to be overexpressed in mucins secreted by patients suffering from various respiratory diseases. To investigate the mechanisms by which airway inflammatory agents mediate the expression of sialyl-Lewis x epitopes and MUC5AC mucin, we examined the effects of tumor necrosis factor (TNF)-α and epidermal growth factor (EGF) in the human lung carcinoma cell line, NCI-H292. Basal expression levels of hST3GalIV, FUT3 and C2/4GnT mRNA, involved in the biosynthesis of sialyl-Lewis x, were higher than those of other glycosyltransferases in NCI-H292 cells. TNF-α induced expression of hST3GalIV, FUT3, C2/4GnT and MUC5AC mRNAs in NCI-H292 cells. When cells were pretreated with U73122, a phosphatidylinositol- phospholipase C (PI-PLC) inhibitor, the expression of these glycosyltransferase mRNAs was suppressed. Treating cells with EGF induced the down-regulation of these glycosyltransferase mRNAs and sialyl-Lewis x epitopes, while inducing an increase in expression of MUC5AC mRNA. These EGF-mediated effects on the glycosyltransferase and MUC5AC mRNAs were blocked when cells were first exposed to AG1478, an EGF receptor tyrosine kinase inhibitor. These findings suggest that the expression of sialyl-Lewis x epitopes, which is regulated separately from the expression of MUC5AC protein, may be controlled through pathways such as the EGF receptor tyrosine kinase and PI-PLC signaling cascades in NCI-H292 cells.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalGlycoconjugate Journal
Volume22
Issue number1-2
DOIs
Publication statusPublished - 2005 Feb
Externally publishedYes

Fingerprint

Epidermal Growth Factor
Epitopes
Glycosyltransferases
Tumor Necrosis Factor-alpha
Cells
Carcinoma
Cell Line
Messenger RNA
Type C Phospholipases
Mucins
Phosphatidylinositols
Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Pulmonary diseases
Biosynthesis
5-acetylneuraminyl-(2-3)-galactosyl-(1-4)-(fucopyranosyl-(1-3))-N-acetylglucosamine
Proteins
Down-Regulation
Lung

Keywords

  • Fucosyltransferase
  • MUC5AC
  • Sialyl-Lewis x
  • Sialyltransferase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of sialyl-Lewis x epitope expression by TNF-α and EGF in an airway carcinoma cell line. / Ishibashi, Yuji; Inouye, Yoshio; Okano, Teruo; Taniguchi, Akiyoshi.

In: Glycoconjugate Journal, Vol. 22, No. 1-2, 02.2005, p. 53-62.

Research output: Contribution to journalArticle

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