Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1

Naoya Yamashita, Asa Morita, Yutaka Uchida, Fumio Nakamura, Hiroshi Usui, Toshio Ohshima, Masahiko Taniguchi, Jérôme Honnorat, Nicole Thomasset, Kohtaro Takei, Takuya Takahashi, Pappachan Kolattukudy, Yoshio Goshima

    Research output: Contribution to journalArticle

    84 Citations (Scopus)

    Abstract

    Collapsin response mediator protein 1 (CRMP1) is one of the CRMP family members that mediates signal transduction of axonal guidance and neuronal migration. We show here evidence that CRMP1 is involved in semaphorin3A (Sema3A)-induced spine development in the cerebral cortex. In the cultured cortical neurons from crmp1+/- mice, Sema3A increased the density of clusters of synapsin I and postsynaptic density-95, but this increase was markedly attenuated in crmp1-/- mice. This attenuation was also seen in cyclin-dependent kinase 5 (cdk5)-/- neurons. Furthermore, the introduction of wild-type CRMP1 but not CRMP1-T509A/S522A, (Thr 509 and Ser 522 were replaced by Ala), a mutant that cannot be phosphorylated by Cdk5, into crmp1-/- neurons rescued the defect in Sema3A responsiveness. The Golgi-impregnation method showed that the crmp1-/- layer V cortical neurons showed a lower density of synaptic bouton-like structures and that this phenotype had genetic interaction with sema3A. These findings suggest that Sema3A-induced spine development is regulated by phosphorylation of CRMP1 by Cdk5.

    Original languageEnglish
    Pages (from-to)12546-12554
    Number of pages9
    JournalJournal of Neuroscience
    Volume27
    Issue number46
    DOIs
    Publication statusPublished - 2007 Nov 14

    Fingerprint

    Cyclin-Dependent Kinase 5
    Spine
    Phosphorylation
    Neurons
    Semaphorin-3A
    Synapsins
    Post-Synaptic Density
    Presynaptic Terminals
    Cerebral Cortex
    Signal Transduction
    collapsin response mediator protein-1
    Phenotype

    Keywords

    • Cdk5
    • Cerebral cortex
    • CRMP
    • PSD-95
    • Sema3A
    • Spine
    • Synapsin I

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1. / Yamashita, Naoya; Morita, Asa; Uchida, Yutaka; Nakamura, Fumio; Usui, Hiroshi; Ohshima, Toshio; Taniguchi, Masahiko; Honnorat, Jérôme; Thomasset, Nicole; Takei, Kohtaro; Takahashi, Takuya; Kolattukudy, Pappachan; Goshima, Yoshio.

    In: Journal of Neuroscience, Vol. 27, No. 46, 14.11.2007, p. 12546-12554.

    Research output: Contribution to journalArticle

    Yamashita, N, Morita, A, Uchida, Y, Nakamura, F, Usui, H, Ohshima, T, Taniguchi, M, Honnorat, J, Thomasset, N, Takei, K, Takahashi, T, Kolattukudy, P & Goshima, Y 2007, 'Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1', Journal of Neuroscience, vol. 27, no. 46, pp. 12546-12554. https://doi.org/10.1523/JNEUROSCI.3463-07.2007
    Yamashita, Naoya ; Morita, Asa ; Uchida, Yutaka ; Nakamura, Fumio ; Usui, Hiroshi ; Ohshima, Toshio ; Taniguchi, Masahiko ; Honnorat, Jérôme ; Thomasset, Nicole ; Takei, Kohtaro ; Takahashi, Takuya ; Kolattukudy, Pappachan ; Goshima, Yoshio. / Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1. In: Journal of Neuroscience. 2007 ; Vol. 27, No. 46. pp. 12546-12554.
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