Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins

Shotaro Kishikawa, Takehide Murata, Hiromichi Kimura, Kunio Shiota, Kazunari K. Yokoyama

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The Sp family is a family of transcription factors that bind to cis-elements in the promoter regions of various genes. Regulation of transcription by Sp proteins is based on interactions between a GC-rich binding site (GGGCGG) in DNA and C-terminal zinc finger motifs in the proteins. In this study, we characterized the GC-rich promoter of the gene for the DNA methyltransferase (Dnmt1) that is responsible for methylation of cytosine residues in mammals and plays a role in gene silencing. We found that a cis-element (nucleotides -161 to -147) was essential for the expression of the mouse gene for Dnmt1. DNA-binding assays indicated that transcription factors Sp1 and Sp3 bound to the same cis-element in this region in a dose-dependent manner. In Drosophila SL2 cells, which lack the Sp family of transcription factors, forced expression of Sp1 or Sp3 enhanced transcription from the Dnmt1 promoter. Stimulation by Sp1 and Sp3 were independent phenomena. Furthermore, cotransfection reporter assays with a p300-expression plasmid revealed the activation of the promoter of the Dnmt1 gene in the presence of Sp3. The transcriptional coactivator p300 interacted with Sp3 in vivo and in vitro. Our results indicate that expression of the Dnmt1 gene is controled by Sp1 and Sp3 and that p300 is involved in the activation by Sp3.

Original languageEnglish
Pages (from-to)2961-2970
Number of pages10
JournalEuropean Journal of Biochemistry
Volume269
Issue number12
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Zinc Fingers
Transcription
Zinc
Genes
DNA
Sp Transcription Factors
Sp3 Transcription Factor
Sp1 Transcription Factor
Gene Expression
Proteins
Cytosine
Methyltransferases
Gene Silencing
Genetic Promoter Regions
Methylation
Drosophila
Mammals
Assays
Plasmids
Transcription Factors

Keywords

  • Activation of transcription
  • Dnmt1 gene
  • p300
  • Sp1
  • Sp3

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins. / Kishikawa, Shotaro; Murata, Takehide; Kimura, Hiromichi; Shiota, Kunio; Yokoyama, Kazunari K.

In: European Journal of Biochemistry, Vol. 269, No. 12, 2002, p. 2961-2970.

Research output: Contribution to journalArticle

Kishikawa, Shotaro ; Murata, Takehide ; Kimura, Hiromichi ; Shiota, Kunio ; Yokoyama, Kazunari K. / Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins. In: European Journal of Biochemistry. 2002 ; Vol. 269, No. 12. pp. 2961-2970.
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