Relation between effective charge numbers and signals caused by protein adsorption on field effect transistor detection

Sho Hideshima, T. Nakamura, Shigeki Kuroiwa, Tetsuya Osaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes a practical method for predicting signals of an adsorbed protein detected by field effect transistors (FETs). The FETs detect the change of the surface charge caused by protein adsorption. The sensing signals strongly depend on an ionic strength of the buffer solution. The prediction of signals is useful for optimizing the strength. A calculation method using three-dimensional conformation structure of proteins was proposed for the prediction tool in the latest paper citing biotin-avidin interaction as an example. To verify availability of the method for alternative adsorbed protein, an adsorption model of streptavidin, which also has a binding ability to biotin, was investigated. Streptavidin has little charge in a buffer solution at pH 7.4 calculated from the method. The results suggest that the signals caused by adsorption of streptavidin were barely detectable using a biotinylated FET.

Original languageEnglish
Title of host publicationECS Transactions
Pages121-124
Number of pages4
Volume35
Edition7
DOIs
Publication statusPublished - 2011
EventBioelectronics, Biointerfaces, and Biomedical Applications 4 - 219th ECS Meeting - Montreal, QC
Duration: 2011 May 12011 May 6

Other

OtherBioelectronics, Biointerfaces, and Biomedical Applications 4 - 219th ECS Meeting
CityMontreal, QC
Period11/5/111/5/6

Fingerprint

Field effect transistors
Proteins
Adsorption
Surface charge
Ionic strength
Conformations
Availability
Biotin

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Relation between effective charge numbers and signals caused by protein adsorption on field effect transistor detection. / Hideshima, Sho; Nakamura, T.; Kuroiwa, Shigeki; Osaka, Tetsuya.

ECS Transactions. Vol. 35 7. ed. 2011. p. 121-124.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hideshima, S, Nakamura, T, Kuroiwa, S & Osaka, T 2011, Relation between effective charge numbers and signals caused by protein adsorption on field effect transistor detection. in ECS Transactions. 7 edn, vol. 35, pp. 121-124, Bioelectronics, Biointerfaces, and Biomedical Applications 4 - 219th ECS Meeting, Montreal, QC, 11/5/1. https://doi.org/10.1149/1.3571984
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