Relationship between energy-dependent macromolecule uptake and transport granules in the endothelial cells affected by wall shear stress

Susumu Kudo, Kenji Ikezawa, Shinji Matsumura, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this study is to reveal (1) the energy dependence of albumin uptake into endothelial cells, and (2) the effect of shear stress on the albumin uptake area and the its content per unit area. The uptake of the fluorescent labeled albumin (tetramethylrhodamine isothiocyanate conjugated albumin, TRITOalbumin) was visualized with a confocal laser scanning microscope. The uptake into the endothelial cells is inhibited completely at 4°C or by 1 μM FCCP, that is a potent energy metabolism inhibitor. This result indicates that the albumin uptake is an energy-dependent, active transport. After 48 hour exposure to shear stress to the endothelial cells, the albumin uptake area and the albumin content per unit area were changed. At 10 dyn/cm2, at 5 μm the uptake area increases by 363% and the albumin content per unit area increases by 192%. At 60 dyn/cm2, at 3 μm the uptake area decreases by 21% and the albumin content per unit area decreases by 54%. It is, therefore, considered that the effect of shear stress on the uptake area is more influential than that on the albumin content per unit area. We conclude that endothelial cells affected by sher stress change the albumin uptake function and especially the cells change the uptake area.

Original languageEnglish
Pages (from-to)2123-2131
Number of pages9
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume64
Issue number623
Publication statusPublished - 1998 Jul

Fingerprint

Endothelial cells
Macromolecules
albumins
macromolecules
shear stress
Shear stress
energy
Microscopes
Scanning
Lasers
metabolism
inhibitors
microscopes

Keywords

  • Active transport
  • Albumin
  • Bio-fluid mechanics
  • Biological engineering
  • Compartment
  • Endothelial cell
  • Macromolecule uptake
  • Shear flow

ASJC Scopus subject areas

  • Mechanical Engineering
  • Condensed Matter Physics

Cite this

Relationship between energy-dependent macromolecule uptake and transport granules in the endothelial cells affected by wall shear stress. / Kudo, Susumu; Ikezawa, Kenji; Matsumura, Shinji; Ikeda, Mariko; Oka, Kotaro; Tanishita, Kazuo.

In: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, Vol. 64, No. 623, 07.1998, p. 2123-2131.

Research output: Contribution to journalArticle

@article{2cc232ed1bd948c1b0941a1bb3a94e11,
title = "Relationship between energy-dependent macromolecule uptake and transport granules in the endothelial cells affected by wall shear stress",
abstract = "The purpose of this study is to reveal (1) the energy dependence of albumin uptake into endothelial cells, and (2) the effect of shear stress on the albumin uptake area and the its content per unit area. The uptake of the fluorescent labeled albumin (tetramethylrhodamine isothiocyanate conjugated albumin, TRITOalbumin) was visualized with a confocal laser scanning microscope. The uptake into the endothelial cells is inhibited completely at 4°C or by 1 μM FCCP, that is a potent energy metabolism inhibitor. This result indicates that the albumin uptake is an energy-dependent, active transport. After 48 hour exposure to shear stress to the endothelial cells, the albumin uptake area and the albumin content per unit area were changed. At 10 dyn/cm2, at 5 μm the uptake area increases by 363{\%} and the albumin content per unit area increases by 192{\%}. At 60 dyn/cm2, at 3 μm the uptake area decreases by 21{\%} and the albumin content per unit area decreases by 54{\%}. It is, therefore, considered that the effect of shear stress on the uptake area is more influential than that on the albumin content per unit area. We conclude that endothelial cells affected by sher stress change the albumin uptake function and especially the cells change the uptake area.",
keywords = "Active transport, Albumin, Bio-fluid mechanics, Biological engineering, Compartment, Endothelial cell, Macromolecule uptake, Shear flow",
author = "Susumu Kudo and Kenji Ikezawa and Shinji Matsumura and Mariko Ikeda and Kotaro Oka and Kazuo Tanishita",
year = "1998",
month = "7",
language = "English",
volume = "64",
pages = "2123--2131",
journal = "Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B",
issn = "0387-5016",
publisher = "Japan Society of Mechanical Engineers",
number = "623",

}

TY - JOUR

T1 - Relationship between energy-dependent macromolecule uptake and transport granules in the endothelial cells affected by wall shear stress

AU - Kudo, Susumu

AU - Ikezawa, Kenji

AU - Matsumura, Shinji

AU - Ikeda, Mariko

AU - Oka, Kotaro

AU - Tanishita, Kazuo

PY - 1998/7

Y1 - 1998/7

N2 - The purpose of this study is to reveal (1) the energy dependence of albumin uptake into endothelial cells, and (2) the effect of shear stress on the albumin uptake area and the its content per unit area. The uptake of the fluorescent labeled albumin (tetramethylrhodamine isothiocyanate conjugated albumin, TRITOalbumin) was visualized with a confocal laser scanning microscope. The uptake into the endothelial cells is inhibited completely at 4°C or by 1 μM FCCP, that is a potent energy metabolism inhibitor. This result indicates that the albumin uptake is an energy-dependent, active transport. After 48 hour exposure to shear stress to the endothelial cells, the albumin uptake area and the albumin content per unit area were changed. At 10 dyn/cm2, at 5 μm the uptake area increases by 363% and the albumin content per unit area increases by 192%. At 60 dyn/cm2, at 3 μm the uptake area decreases by 21% and the albumin content per unit area decreases by 54%. It is, therefore, considered that the effect of shear stress on the uptake area is more influential than that on the albumin content per unit area. We conclude that endothelial cells affected by sher stress change the albumin uptake function and especially the cells change the uptake area.

AB - The purpose of this study is to reveal (1) the energy dependence of albumin uptake into endothelial cells, and (2) the effect of shear stress on the albumin uptake area and the its content per unit area. The uptake of the fluorescent labeled albumin (tetramethylrhodamine isothiocyanate conjugated albumin, TRITOalbumin) was visualized with a confocal laser scanning microscope. The uptake into the endothelial cells is inhibited completely at 4°C or by 1 μM FCCP, that is a potent energy metabolism inhibitor. This result indicates that the albumin uptake is an energy-dependent, active transport. After 48 hour exposure to shear stress to the endothelial cells, the albumin uptake area and the albumin content per unit area were changed. At 10 dyn/cm2, at 5 μm the uptake area increases by 363% and the albumin content per unit area increases by 192%. At 60 dyn/cm2, at 3 μm the uptake area decreases by 21% and the albumin content per unit area decreases by 54%. It is, therefore, considered that the effect of shear stress on the uptake area is more influential than that on the albumin content per unit area. We conclude that endothelial cells affected by sher stress change the albumin uptake function and especially the cells change the uptake area.

KW - Active transport

KW - Albumin

KW - Bio-fluid mechanics

KW - Biological engineering

KW - Compartment

KW - Endothelial cell

KW - Macromolecule uptake

KW - Shear flow

UR - http://www.scopus.com/inward/record.url?scp=71249136947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71249136947&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:71249136947

VL - 64

SP - 2123

EP - 2131

JO - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

JF - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

SN - 0387-5016

IS - 623

ER -