Relationship between stable carbon and nitrogen isotope ratios of humic acids extracted from Andisols and non-Andisols

Naoya Katsumi, Koyo Yonebayashi, Nobuhide Fujitake, Masanori Okazaki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To relate the isotopic composition of soil humic acids (HAs) to their source plants and their degree of humification (as indicated by the degree of darkening), as well as to soil type, we determined the stable isotope ratios for carbon (δ13C) and nitrogen (δ15N) of HAs extracted from 26 virgin soil samples (14 Andisols and 12 non-Andisols) and from leaf samples from ten C3 plants and seven C4 plants. The high absorption coefficients (estimated at 600nm for 1% solutions), low hydrogen and nitrogen contents, low H/C ratios, low alkyl C and O-alkyl C contents, high O/H and C/N ratios, high aryl C contents, and high aromaticity of the Andisol HAs indicated a high degree of humification. The δ13C and δ15N values of the non-Andisol HAs increased along with the progression of humification of C3 plant-derived HAs. A significant correlation was observed between the δ15N and δ13C values of the non-Andisol HAs, but not for the Andisol HAs. Most of the Andisol HA δ13C values were higher than the highest non-Andisol HA δ13C value, and δ13C values were distributed between the δ13C values for the C3 and C4 plants. The contributions of C4 plant-derived carbon to total Andisol HA carbon (CRC4) are usually calculated on the assumption that the δ13C values are not substantially altered during the decomposition of plant materials and the formation of soil organic matter. However, if 13C enrichment occurs during organic matter decomposition, CRC4 will be overestimated. Therefore, we suggest a new method to calculate CRC4 values of Andisol HAs with a correction for isotopic fractionation associated with microbial degradation; the corrected CRC4 values ranged from -5% to 58% and were approximately 22% lower than the uncorrected values.

Original languageEnglish
Pages (from-to)214-221
Number of pages8
JournalCatena
Volume127
DOIs
Publication statusPublished - 2015 Apr 1
Externally publishedYes

Fingerprint

Andisol
nitrogen isotope
humic acid
carbon isotope
stable isotope
C4 plant
C3 plant
humification
carbon
decomposition
nitrogen
isotopic fractionation
absorption coefficient
soil organic matter
soil type
isotopic composition
soil

Keywords

  • Andisol
  • Humic acid
  • Non-Andisol
  • Stable carbon isotope ratio
  • Stable nitrogen isotope ratio

ASJC Scopus subject areas

  • Earth-Surface Processes

Cite this

Relationship between stable carbon and nitrogen isotope ratios of humic acids extracted from Andisols and non-Andisols. / Katsumi, Naoya; Yonebayashi, Koyo; Fujitake, Nobuhide; Okazaki, Masanori.

In: Catena, Vol. 127, 01.04.2015, p. 214-221.

Research output: Contribution to journalArticle

Katsumi, Naoya ; Yonebayashi, Koyo ; Fujitake, Nobuhide ; Okazaki, Masanori. / Relationship between stable carbon and nitrogen isotope ratios of humic acids extracted from Andisols and non-Andisols. In: Catena. 2015 ; Vol. 127. pp. 214-221.
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AB - To relate the isotopic composition of soil humic acids (HAs) to their source plants and their degree of humification (as indicated by the degree of darkening), as well as to soil type, we determined the stable isotope ratios for carbon (δ13C) and nitrogen (δ15N) of HAs extracted from 26 virgin soil samples (14 Andisols and 12 non-Andisols) and from leaf samples from ten C3 plants and seven C4 plants. The high absorption coefficients (estimated at 600nm for 1% solutions), low hydrogen and nitrogen contents, low H/C ratios, low alkyl C and O-alkyl C contents, high O/H and C/N ratios, high aryl C contents, and high aromaticity of the Andisol HAs indicated a high degree of humification. The δ13C and δ15N values of the non-Andisol HAs increased along with the progression of humification of C3 plant-derived HAs. A significant correlation was observed between the δ15N and δ13C values of the non-Andisol HAs, but not for the Andisol HAs. Most of the Andisol HA δ13C values were higher than the highest non-Andisol HA δ13C value, and δ13C values were distributed between the δ13C values for the C3 and C4 plants. The contributions of C4 plant-derived carbon to total Andisol HA carbon (CRC4) are usually calculated on the assumption that the δ13C values are not substantially altered during the decomposition of plant materials and the formation of soil organic matter. However, if 13C enrichment occurs during organic matter decomposition, CRC4 will be overestimated. Therefore, we suggest a new method to calculate CRC4 values of Andisol HAs with a correction for isotopic fractionation associated with microbial degradation; the corrected CRC4 values ranged from -5% to 58% and were approximately 22% lower than the uncorrected values.

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