Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T

Shinichi MacHida, Ryota Hayashida, Motoki Takaku, Atsuhiko Fukuto, Jiying Sun, Aiko Kinomura, Satoshi Tashiro, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Linker histones bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration. Somatic and germ cell-specific linker histone subtypes have been identified and may have distinct functions. In this study, we reconstituted polynucleosomes containing human histones H1.2 and H1T, as representative somatic and germ cell-specific linker histones, respectively, and found that H1T forms less compacted chromatin, as compared to H1.2. An in vitro homologous pairing assay revealed that H1T weakly inhibited RAD51/RAD54-mediated homologous pairing in chromatin, although the somatic H1 subtypes, H1.0, H1.1, H1.2, H1.3, H1.4, and H1.5, substantially suppressed it. An in vivo recombination assay revealed that H1T overproduction minimally affected the recombination frequency, but significant suppression was observed when H1.2 was overproduced in human cells. These results suggested that the testis-specific linker histone, H1T, possesses a specific function to produce the chromatin architecture required for proper chromosome regulation, such as homologous recombination.

    Original languageEnglish
    Pages (from-to)637-646
    Number of pages10
    JournalBiochemistry
    Volume55
    Issue number4
    DOIs
    Publication statusPublished - 2016 Feb 2

    Fingerprint

    Histones
    Chromatin
    Testis
    Nucleosomes
    Cells
    Germ Cells
    Genetic Recombination
    Assays
    Homologous Recombination
    Chromosomes

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    MacHida, S., Hayashida, R., Takaku, M., Fukuto, A., Sun, J., Kinomura, A., ... Kurumizaka, H. (2016). Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T. Biochemistry, 55(4), 637-646. https://doi.org/10.1021/acs.biochem.5b01126

    Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T. / MacHida, Shinichi; Hayashida, Ryota; Takaku, Motoki; Fukuto, Atsuhiko; Sun, Jiying; Kinomura, Aiko; Tashiro, Satoshi; Kurumizaka, Hitoshi.

    In: Biochemistry, Vol. 55, No. 4, 02.02.2016, p. 637-646.

    Research output: Contribution to journalArticle

    MacHida, S, Hayashida, R, Takaku, M, Fukuto, A, Sun, J, Kinomura, A, Tashiro, S & Kurumizaka, H 2016, 'Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T', Biochemistry, vol. 55, no. 4, pp. 637-646. https://doi.org/10.1021/acs.biochem.5b01126
    MacHida, Shinichi ; Hayashida, Ryota ; Takaku, Motoki ; Fukuto, Atsuhiko ; Sun, Jiying ; Kinomura, Aiko ; Tashiro, Satoshi ; Kurumizaka, Hitoshi. / Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T. In: Biochemistry. 2016 ; Vol. 55, No. 4. pp. 637-646.
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