Release of α-subunit of glycoprotein hormones from the bullfrog pituitary: Possible effect of α-subunit on prolactin cell function

Atsuko Oguchi, Shigeyasu Tanaka, Kazutoshi Yamamoto, Sakae Kikuyama

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    Abstract

    On the basis of our previous finding that prolactin (PRL) cells of the bullfrog (Rana catesbeiana) contain immunoreactive α-subunit of pituitary glycoprotein hormones, experiments were conducted to see whether the immunoreactive α-subunit is released from PRL cells. Cell immunoblot analysis revealed that approximately 10% of cells that released immunoreactive PRL also released immunoreactive α-subunit. Western blot analysis of the culture media revealed that a considerable amount of the free form of α-subunit was released from the dispersed anterior pituitary cells. Moreover, it was found that addition of α-subunit to the culture medium enhanced PRL release. The results raise the possibility that α-subunit secreted by PRL cells acts as an autocrine and/or paracrine factor.

    Original languageEnglish
    Pages (from-to)141-146
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume102
    Issue number1
    DOIs
    Publication statusPublished - 1996 Apr

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    ASJC Scopus subject areas

    • Endocrinology

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