Release of α-subunit of glycoprotein hormones from the bullfrog pituitary: Possible effect of α-subunit on prolactin cell function

Atsuko Oguchi, Shigeyasu Tanaka, Kazutoshi Yamamoto, Sakae Kikuyama

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    On the basis of our previous finding that prolactin (PRL) cells of the bullfrog (Rana catesbeiana) contain immunoreactive α-subunit of pituitary glycoprotein hormones, experiments were conducted to see whether the immunoreactive α-subunit is released from PRL cells. Cell immunoblot analysis revealed that approximately 10% of cells that released immunoreactive PRL also released immunoreactive α-subunit. Western blot analysis of the culture media revealed that a considerable amount of the free form of α-subunit was released from the dispersed anterior pituitary cells. Moreover, it was found that addition of α-subunit to the culture medium enhanced PRL release. The results raise the possibility that α-subunit secreted by PRL cells acts as an autocrine and/or paracrine factor.

    Original languageEnglish
    Pages (from-to)141-146
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume102
    Issue number1
    DOIs
    Publication statusPublished - 1996 Apr

    Fingerprint

    Rana catesbeiana
    Pituitary Hormones
    prolactin
    Prolactin
    glycoproteins
    Glycoproteins
    hormones
    cells
    Culture Media
    culture media
    Lithobates catesbeianus
    anterior pituitary
    Western blotting
    Western Blotting

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Release of α-subunit of glycoprotein hormones from the bullfrog pituitary : Possible effect of α-subunit on prolactin cell function. / Oguchi, Atsuko; Tanaka, Shigeyasu; Yamamoto, Kazutoshi; Kikuyama, Sakae.

    In: General and Comparative Endocrinology, Vol. 102, No. 1, 04.1996, p. 141-146.

    Research output: Contribution to journalArticle

    Oguchi, Atsuko ; Tanaka, Shigeyasu ; Yamamoto, Kazutoshi ; Kikuyama, Sakae. / Release of α-subunit of glycoprotein hormones from the bullfrog pituitary : Possible effect of α-subunit on prolactin cell function. In: General and Comparative Endocrinology. 1996 ; Vol. 102, No. 1. pp. 141-146.
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