Reliable imaging of ATP in living budding and fission yeast

Masak Takaine, Masaru Ueno, Kenji Kitamura, Hiromi Imamura, Satoshi Yoshida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Adenosine triphosphate (ATP) is a main metabolite essential for all living organisms. However, our understanding of ATP dynamics within a single living cell is very limited. Here, we optimized the ATP-biosensor QUEEN and monitored the dynamics of ATP with good spatial and temporal resolution in living yeasts.We found stable maintenance of ATP concentration in wild-type yeasts, regardless of carbon sources or cell cycle stages, suggesting that mechanism exists to maintain ATP at a specific concentration. We further found that ATP concentration is not necessarily an indicator of metabolic activity, as there is no clear correlation between ATP level and growth rates. During fission yeast meiosis, we found a reduction in ATP levels, suggesting that ATP homeostasis is controlled by differentiation. The use of QUEEN in yeasts offers an easy and reliable assay for ATP dynamicity and will answer several unaddressed questions about cellular metabolism in eukaryotes.

Original languageEnglish
Article number230649
JournalJournal of Cell Science
Volume132
Issue number8
DOIs
Publication statusPublished - 2019 Apr 1

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Saccharomycetales
Schizosaccharomyces
Adenosine Triphosphate
Yeasts
Carbon Cycle
Meiosis
Biosensing Techniques
Eukaryota
Cell Cycle
Homeostasis
Maintenance

Keywords

  • ATP
  • Carbon metabolism
  • Homeostasis
  • Meiosis
  • Metabolism
  • Mitochondria
  • Yeast

ASJC Scopus subject areas

  • Cell Biology

Cite this

Reliable imaging of ATP in living budding and fission yeast. / Takaine, Masak; Ueno, Masaru; Kitamura, Kenji; Imamura, Hiromi; Yoshida, Satoshi.

In: Journal of Cell Science, Vol. 132, No. 8, 230649, 01.04.2019.

Research output: Contribution to journalArticle

Takaine, Masak ; Ueno, Masaru ; Kitamura, Kenji ; Imamura, Hiromi ; Yoshida, Satoshi. / Reliable imaging of ATP in living budding and fission yeast. In: Journal of Cell Science. 2019 ; Vol. 132, No. 8.
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