Remarked suppression of Aβ42 protomer–protomer dissociation reaction elucidated by molecular dynamics simulation

Ikuo Kurisaki*, Shigenori Tanaka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Multimeric protein complexes are molecular apparatuses to regulate biological systems and often determine their fate. Among proteins forming such molecular assemblies, amyloid proteins have drawn attention over a half-century since amyloid fibril formation of these proteins is supposed to be a common pathogenic cause for neurodegenerative diseases. This process is triggered by the accumulation of fibril-like aggregates, while the microscopic mechanisms are mostly elusive due to technical limitation of experimental methodologies in individually observing each of diverse aggregate species in the aqueous solution. We then addressed this problem by employing atomistic molecular dynamics simulations for the paradigmatic amyloid protein, amyloid-β (Aβ42). Seven different dimeric forms of oligomeric Aβ42 fibril-like aggregate in aqueous solution, ranging from tetramer to decamer, were considered. We found additive effects of the size of these fibril-like aggregates on their thermodynamic stability and have clarified kinetic suppression of protomer-protomer dissociation reactions at and beyond the point of pentamer dimer formation. This observation was obtained from the specific combination of the Aβ42 protomer structure and the physicochemical condition that we here examined, while it is worthwhile to recall that several amyloid fibrils take dimeric forms of their protomers. We could thus conclude that the stable formation of fibril-like protomer dimer should be involved in a turning point where rapid growth of amyloid fibrils is triggered.

Original languageEnglish
Pages (from-to)1367-1375
Number of pages9
JournalProteins: Structure, Function and Bioinformatics
Volume90
Issue number7
DOIs
Publication statusPublished - 2022 Jul
Externally publishedYes

Keywords

  • amyloid fibril formation
  • amyloid-β (1–42)
  • molecular dynamics simulations
  • protein (dis)assembly
  • protein aggregate
  • thermodynamic stability

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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