Removal of urea from water using urease-immobilized fibers

Mai Sugiyama, Kohsuke Ikeda, Daisuke Umeno, Kyoichi Saito, Takashi Kikuchi, Kiyoto Ando

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Dimethylaminoethyl methacrylate (DMAEMA was graft-polymerized at a density of 1.7 mmol/g onto a 6-nylon fiberby radiation-induced graft polymerization. Urease was bound to the resultant anion-exchange fiberby electrostatic interaction. After crosslinking with transglutaminase, 80% of the bound urease was immobilized at a density of 41 mg/g of the fiber. Urea in water was quantitatively hydrolyzed during the permeation of a 0.20 mg/L urea solution through the urease-immobilized-fiber-packed bed with a diameter of 5.5 mm and a height of 27 mm in the residence time range of 12-180 s. The activity of immobilized urease did not deteriorate after repeated use of the fiber, i.e., during reaction and storage.

Original languageEnglish
Pages (from-to)509-513
Number of pages5
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume46
Issue number7
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • 6-nylon fiber
  • Dimethylaminoethyl methacrylate
  • Electron-beam-induced graft polymerization
  • Urea hydrolysis
  • Urease immobilization

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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