Abstract
Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.
| Original language | English |
|---|---|
| Pages (from-to) | 834-840 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 31 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure. / Matsunaga, Tadashi; Higashijima, Michio; Sulaswatty, Anny; Nishimura, Shigeo; Kitamura, Takanori; Tsuji, Masao; Kawaguchi, Toshio.
In: Biotechnology and Bioengineering, Vol. 31, No. 8, 1988, p. 834-840.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure
AU - Matsunaga, Tadashi
AU - Higashijima, Michio
AU - Sulaswatty, Anny
AU - Nishimura, Shigeo
AU - Kitamura, Takanori
AU - Tsuji, Masao
AU - Kawaguchi, Toshio
PY - 1988
Y1 - 1988
N2 - Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.
AB - Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.
UR - http://www.scopus.com/inward/record.url?scp=0024011944&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024011944&partnerID=8YFLogxK
U2 - 10.1002/bit.260310811
DO - 10.1002/bit.260310811
M3 - Article
AN - SCOPUS:0024011944
VL - 31
SP - 834
EP - 840
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 8
ER -