Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure

Tadashi Matsunaga, Michio Higashijima, Anny Sulaswatty, Shigeo Nishimura, Takanori Kitamura, Masao Tsuji, Toshio Kawaguchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Among various microbial cells examined under screening conditions, Nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. Here NH4Cl as well as amino acids were used as an amino donor for phenylalanine production. The phenylalanine production rate increased with increasing hydrogen pressure. The specific activity of phenylalanine dehydrogenase was increased by culturing N. opaca cells in nutrient broth containing 0.3% phenylalanine. As a result, the phenylalanine production rate increased from 0.69 to 4.4 μmol/min g dry cells. Immobilized cells were activated in nutrient broth containing ZnCl2 before phenylalanine production. Phenylalanine dehydrogenase activity and cell number in the gel increased with increasing incubation time, and the maximum phenylalanine dehydrogenase activity was obtained at 36 h incubation. Then, phenylalanine was produced from phenylpyruvate, NH4Cl, and 100 atm H2 with the activated immobilized cells. The rate of phenylalanine production was 0.24 μmol/min cm3 gel. The conversion of phenylpyruvate to phenylalanine was 82%. Immobilized cells retained 76% of the initial phenylalanine production rate after 10 h reactions were repeated 11 times with two intervening reactivations.

Original languageEnglish
Pages (from-to)834-840
Number of pages7
JournalBiotechnology and Bioengineering
Volume31
Issue number8
DOIs
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Nocardia
Immobilized Cells
Phenylalanine
Hydrogen
Cells
Pressure
Nutrients
Gels
phenylpyruvic acid
Food
Amino acids
Screening
Cell Count
Amino Acids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure. / Matsunaga, Tadashi; Higashijima, Michio; Sulaswatty, Anny; Nishimura, Shigeo; Kitamura, Takanori; Tsuji, Masao; Kawaguchi, Toshio.

In: Biotechnology and Bioengineering, Vol. 31, No. 8, 1988, p. 834-840.

Research output: Contribution to journalArticle

Matsunaga, Tadashi ; Higashijima, Michio ; Sulaswatty, Anny ; Nishimura, Shigeo ; Kitamura, Takanori ; Tsuji, Masao ; Kawaguchi, Toshio. / Repeated batch production of L‐phenylalanine from phenylpyruvate and NH4Cl by immobilized cells of Nocardia opaca under hydrogen high pressure. In: Biotechnology and Bioengineering. 1988 ; Vol. 31, No. 8. pp. 834-840.
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AU - Nishimura, Shigeo

AU - Kitamura, Takanori

AU - Tsuji, Masao

AU - Kawaguchi, Toshio

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