Repression of the activities of two extracytoplasmic function σ factors, σM and σV, of Bacillus subtilis by glucolipids in Escherichia coli cells

Takahiro Seki, Ryota Mineshima, Michihiro Hashimoto, Kouji Matsumoto, Hiroshi Hara*, Satoshi Matsuoka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Extracytoplasmic function (ECF) σ factors respond to environmental stresses and regulate numerous genes required for adaptation. Under normal growth conditions, the ECF σ factors are sequestered by transmembrane anti-σ factor proteins, from which they are released under stress conditions. In Bacillus subtilis ugtP null mutant cells, which lack glucolipids, three of the seven ECF σ factors, σM, σV and σX, are activated. The Escherichia coli cell membrane does not contain glucolipids. When the genes for these three ECF σ and anti-σ factors were introduced into E. coli cells, expression of lacZ fused to the ECF σ factor-regulated promoters indicated ECF σ factor activity. Additional expression of the ugtP gene in these E. coli cells led to the synthesis of small amounts of glucolipids, and the activities of σM and σV were repressed, but the activity of σX was unaffected. It is likely that glucolipids directly influence anti-σM and anti-σV factors by stabilizing conformations that sequester the respective ECF σ factors.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalGenes and Genetic Systems
Volume90
Issue number2
DOIs
Publication statusPublished - 2015 Sep 18
Externally publishedYes

Keywords

  • Anti-σ factor
  • Bacillus subtilis
  • ECF σ factor
  • Escherichia coli
  • Glucolipid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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