Reproducibility of fluorescent expression from engineered biological constructs in E. coli

iGEM Interlab Study Contributors

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.

Original languageEnglish
Article numbere0150182
JournalPLoS One
Volume11
Issue number3
DOIs
Publication statusPublished - 2016 Mar 1
Externally publishedYes

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reproducibility
Escherichia coli
Fluorescence
promoter regions
fluorescence
Synthetic Biology
synthetic biology
host strains
Reproducibility of Results

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Reproducibility of fluorescent expression from engineered biological constructs in E. coli. / iGEM Interlab Study Contributors.

In: PLoS One, Vol. 11, No. 3, e0150182, 01.03.2016.

Research output: Contribution to journalArticle

iGEM Interlab Study Contributors. / Reproducibility of fluorescent expression from engineered biological constructs in E. coli. In: PLoS One. 2016 ; Vol. 11, No. 3.
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