Reproducible subcutaneous transplantation of cell sheets into recipient mice

Haruko Obokata, Masayuki Yamato, Satoshi Tsuneda, Teruo Okano

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Perfecting tissue engineering and cell sheet transplantation is an important step toward realizing regenerative medicine and is a growing area of research. Before being applied to clinical settings, it is important that these approaches are evaluated in vivo. Here we provide a detailed protocol for handling thin cell sheets, for a simple and highly reproducible subcutaneous transplantation of cell sheets into mice, and for the histological examination of regenerated tissues. Various aspects of transplants can be assessed, such as maintenance, differentiation and proliferation. An emphasis is placed on surgical precision and reproducibility. The resulting consistency between surgeries helps minimize artifacts from surgical variation and therefore enables researchers to not only observe and compare the interactions between host tissues but also to compare transplants among different host animals. A single transplantation can be carried out within ∼10 min.

    Original languageEnglish
    Pages (from-to)1053-1059
    Number of pages7
    JournalNature Protocols
    Volume6
    Issue number7
    DOIs
    Publication statusPublished - 2011 Jun

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    Transplantation (surgical)
    Transplants
    Cell Transplantation
    Tissue
    Regenerative Medicine
    Tissue Engineering
    Tissue engineering
    Artifacts
    Surgery
    Animals
    Transplantation
    Maintenance
    Research Personnel
    Research

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Reproducible subcutaneous transplantation of cell sheets into recipient mice. / Obokata, Haruko; Yamato, Masayuki; Tsuneda, Satoshi; Okano, Teruo.

    In: Nature Protocols, Vol. 6, No. 7, 06.2011, p. 1053-1059.

    Research output: Contribution to journalArticle

    Obokata, Haruko ; Yamato, Masayuki ; Tsuneda, Satoshi ; Okano, Teruo. / Reproducible subcutaneous transplantation of cell sheets into recipient mice. In: Nature Protocols. 2011 ; Vol. 6, No. 7. pp. 1053-1059.
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