Abstract
EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.
| Original language | English |
|---|---|
| Pages (from-to) | 358-362 |
| Number of pages | 5 |
| Journal | Nature |
| Volume | 376 |
| Issue number | 6538 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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Cite this
Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation. / Terada, Yasuhiko; Tatsuka, Masaaki; Jinno, Shigeki; Okayama, Hiroto.
In: Nature, Vol. 376, No. 6538, 1995, p. 358-362.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation
AU - Terada, Yasuhiko
AU - Tatsuka, Masaaki
AU - Jinno, Shigeki
AU - Okayama, Hiroto
PY - 1995
Y1 - 1995
N2 - EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.
AB - EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.
UR - http://www.scopus.com/inward/record.url?scp=0029014496&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029014496&partnerID=8YFLogxK
M3 - Article
C2 - 7630405
AN - SCOPUS:0029014496
VL - 376
SP - 358
EP - 362
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6538
ER -