Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation

Yasuhiko Terada, Masaaki Tatsuka, Shigeki Jinno, Hiroto Okayama

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.

Original languageEnglish
Pages (from-to)358-362
Number of pages5
JournalNature
Volume376
Issue number6538
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

S Phase
Tyrosine
Fibroblasts
Phosphorylation
Cyclin-Dependent Kinase Inhibitor p21
G2 Phase
Ultraviolet Rays
Chromosome Aberrations
DNA Damage
Cell Cycle
Cell Death

ASJC Scopus subject areas

  • General

Cite this

Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation. / Terada, Yasuhiko; Tatsuka, Masaaki; Jinno, Shigeki; Okayama, Hiroto.

In: Nature, Vol. 376, No. 6538, 1995, p. 358-362.

Research output: Contribution to journalArticle

Terada, Y, Tatsuka, M, Jinno, S & Okayama, H 1995, 'Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation', Nature, vol. 376, no. 6538, pp. 358-362.
Terada, Yasuhiko ; Tatsuka, Masaaki ; Jinno, Shigeki ; Okayama, Hiroto. / Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation. In: Nature. 1995 ; Vol. 376, No. 6538. pp. 358-362.
@article{1ad25fe1595041859e141c4fb1369b5b,
title = "Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation",
abstract = "EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.",
author = "Yasuhiko Terada and Masaaki Tatsuka and Shigeki Jinno and Hiroto Okayama",
year = "1995",
language = "English",
volume = "376",
pages = "358--362",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6538",

}

TY - JOUR

T1 - Requirement for tyrosine phosphorylation of Cdk4 in Gl arrest induced by ultraviolet irradiation

AU - Terada, Yasuhiko

AU - Tatsuka, Masaaki

AU - Jinno, Shigeki

AU - Okayama, Hiroto

PY - 1995

Y1 - 1995

N2 - EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.

AB - EXPOSURE to ultraviolet light arrests the function of mammalian fibroblasts in the Gl phase of the cell cycle, as well as the S and G2 phases. Although p21, an inhibitor of cyclin-dependent kinase (Cdk) that is induced by DNA damage may partly account for the arrest in Gl (ref. 1), the mechanism is little understood. Here we show that tyrosine phosphorylation of Cdk4 is required for this arrest. In rat fibroblast, Cdk4 is tyrosine-phosphorylated during Gl progression, and its dephosphorylation is required for S phase. When cells are ultraviolet-irradiated, their arrest in Gl is accompanied by an increase in phosphorylation level. Conversely, cells expressing unphosphorx la table Cdk4F17 fail to arrest in Gl, and suffer significantly elevated chromosomal aberrations and cell death.

UR - http://www.scopus.com/inward/record.url?scp=0029014496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029014496&partnerID=8YFLogxK

M3 - Article

C2 - 7630405

AN - SCOPUS:0029014496

VL - 376

SP - 358

EP - 362

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6538

ER -