Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase

Ryohei Yasuda, Hiroyuki Noji, Masasuke Yoshida, Kazuhiko Kinosita, Hiroyasu Itoh

Research output: Contribution to journalArticle

642 Citations (Scopus)

Abstract

The enzyme F1-ATPase has been shown to be a rotary motor in which the central γ-subunit rotates inside the cylinder made of α3β3 subunits. At low ATP concentrations, the motor rotates in discrete 120° steps, consistent with sequential ATP hydrolysis on the three β-subunits. The mechanism of stepping is unknown. Here we show by high-speed imaging that the 120° step consists of roughly 90° and 30° substeps, each taking only a fraction of a millisecond. ATP binding drives the 90° substep, and the 30° substep is probably driven by release of a hydrolysis product. The two substeps are separated by two reactions of about 1 ms, which together occupy most of the ATP hydrolysis cycle. This scheme probably applies to rotation at full speed (∼130 revolutions per second at saturating ATP) down to occasional stepping at nanomolar ATP concentrations, and supports the binding-change model for ATP synthesis by reverse rotation of F1-ATPase.

Original languageEnglish
Pages (from-to)898-904
Number of pages7
JournalNature
Volume410
Issue number6831
DOIs
Publication statusPublished - 2001 Apr 19
Externally publishedYes

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Proton-Translocating ATPases
Adenosine Triphosphate
Hydrolysis
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Yasuda, R., Noji, H., Yoshida, M., Kinosita, K., & Itoh, H. (2001). Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase. Nature, 410(6831), 898-904. https://doi.org/10.1038/35073513

Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase. / Yasuda, Ryohei; Noji, Hiroyuki; Yoshida, Masasuke; Kinosita, Kazuhiko; Itoh, Hiroyasu.

In: Nature, Vol. 410, No. 6831, 19.04.2001, p. 898-904.

Research output: Contribution to journalArticle

Yasuda, R, Noji, H, Yoshida, M, Kinosita, K & Itoh, H 2001, 'Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase', Nature, vol. 410, no. 6831, pp. 898-904. https://doi.org/10.1038/35073513
Yasuda, Ryohei ; Noji, Hiroyuki ; Yoshida, Masasuke ; Kinosita, Kazuhiko ; Itoh, Hiroyasu. / Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase. In: Nature. 2001 ; Vol. 410, No. 6831. pp. 898-904.
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