Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields

Sunao Katsuki, Yulan Li, Daiki Miyakawa, Ryo Yamada, Nobuaki Onishi, Soowon Lim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the biological effect of intense pulse electric field from the frequency point of view. A pulse-modulated sinusoidal wave as the A narrowband pulsed electric field (nbPEF) allows us to deliver a non-thermal, intense and well-defined electric field in terms of frequency, field strength and deposition energy to biological systems. 10 μs long sinusoidal electric fields with a frequency range between 0.1 and 100 MHz and field strengths of up to 10 kV/cm were applied to HeLa or HeLaS3 cells, which were subsequently analyzed in terms of the morphology and the Ca2+ response. The field with the frequency below a few MHz immediately causes blebs in the external field direction, whereas the morphology does not change apparently in the case of the frequency more than 10 MHz. The intracellular Ca2+ concentration rapidly increased after the exposure to the low frequency field and subsequently decayed exponentially within hundreds of seconds. Inversely, for the high frequency fields, the Ca2+ concentration did not change for seconds after the pulse, but increased gradually in tens to hundreds of seconds. When the Ca2+ channel on the plasma membrane was inhibited, the delayed Ca2+ uptake was suppressed. Our experiment shows that the possibility to activate or impair function of membrane proteins physically by using nbPEF without significant defects of the plasma membrane.

Original languageEnglish
Title of host publication2017 11th European Conference on Antennas and Propagation, EUCAP 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1358-1361
Number of pages4
ISBN (Electronic)9788890701870
DOIs
Publication statusPublished - 2017 May 15
Externally publishedYes
Event11th European Conference on Antennas and Propagation, EUCAP 2017 - Paris, France
Duration: 2017 Mar 192017 Mar 24

Publication series

Name2017 11th European Conference on Antennas and Propagation, EUCAP 2017

Conference

Conference11th European Conference on Antennas and Propagation, EUCAP 2017
CountryFrance
CityParis
Period17/3/1917/3/24

Keywords

  • biological effect
  • blebbing
  • Ca uptake
  • cell mophology
  • narrowband pulsed electric field
  • pore formation
  • TRP channel

ASJC Scopus subject areas

  • Safety Research
  • Computer Networks and Communications
  • Signal Processing
  • Instrumentation

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  • Cite this

    Katsuki, S., Li, Y., Miyakawa, D., Yamada, R., Onishi, N., & Lim, S. (2017). Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields. In 2017 11th European Conference on Antennas and Propagation, EUCAP 2017 (pp. 1358-1361). [7928345] (2017 11th European Conference on Antennas and Propagation, EUCAP 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.23919/EuCAP.2017.7928345