Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus

Reiko Hara, Keiko Wan, Hisanori Wakamatsu, Reiko Aida, Takahiro Moriya, Masashi Akiyama, Shigenobu Shibata

    Research output: Contribution to journalArticle

    399 Citations (Scopus)

    Abstract

    Background: There are two main stimuli that entrain the circadian rhythm, the light-dark cycle (LD) and restricted feeding (RF). Light-induced entrainment requires induction of the Per1 and Per2 genes in the suprachiasmatic nucleus (SCN), the locus of a main oscillator. In this experiment, we determined whether RF resets the expression of circadian clock genes in the mouse liver with or without participation of the SCN. Results: Mice were allowed access to food for 4 h during the daytime (7 h advance of feeding time) under LD or constant darkness (DD). The peaks of mPer1, mPer2, D-site-binding protein (Dbp) and cholesterol 7α-hydroxylase (Cyp7A) mRNA in the liver were advanced 6-12 h after 6 days of RF, whereas those in SCN were unaffected. The advance of mPer expression in the liver by RF was still observed in SCN-lesioned mice. A 7 h advance in the LD cycle advanced the peaks of clock gene expression in both the liver and SCN, whereas, a shift in the LD did not move the phase of the liver clock when the shift was carried out under a fixed RF schedule during the night-time. Conclusions: These results suggest that restricted feeding strongly entrained the expression of circadian clock genes in the liver without the participation of an SCN clock function.

    Original languageEnglish
    Pages (from-to)269-278
    Number of pages10
    JournalGenes to Cells
    Volume6
    Issue number3
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Suprachiasmatic Nucleus
    Photoperiod
    Liver
    Circadian Clocks
    Cholesterol 7-alpha-Hydroxylase
    Genes
    Darkness
    Circadian Rhythm
    Appointments and Schedules
    Carrier Proteins
    Gene Expression
    Light
    Food
    Messenger RNA

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    Cite this

    Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus. / Hara, Reiko; Wan, Keiko; Wakamatsu, Hisanori; Aida, Reiko; Moriya, Takahiro; Akiyama, Masashi; Shibata, Shigenobu.

    In: Genes to Cells, Vol. 6, No. 3, 2001, p. 269-278.

    Research output: Contribution to journalArticle

    Hara, Reiko ; Wan, Keiko ; Wakamatsu, Hisanori ; Aida, Reiko ; Moriya, Takahiro ; Akiyama, Masashi ; Shibata, Shigenobu. / Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus. In: Genes to Cells. 2001 ; Vol. 6, No. 3. pp. 269-278.
    @article{d2e22e5999954401ad08fafbde0eca70,
    title = "Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus",
    abstract = "Background: There are two main stimuli that entrain the circadian rhythm, the light-dark cycle (LD) and restricted feeding (RF). Light-induced entrainment requires induction of the Per1 and Per2 genes in the suprachiasmatic nucleus (SCN), the locus of a main oscillator. In this experiment, we determined whether RF resets the expression of circadian clock genes in the mouse liver with or without participation of the SCN. Results: Mice were allowed access to food for 4 h during the daytime (7 h advance of feeding time) under LD or constant darkness (DD). The peaks of mPer1, mPer2, D-site-binding protein (Dbp) and cholesterol 7α-hydroxylase (Cyp7A) mRNA in the liver were advanced 6-12 h after 6 days of RF, whereas those in SCN were unaffected. The advance of mPer expression in the liver by RF was still observed in SCN-lesioned mice. A 7 h advance in the LD cycle advanced the peaks of clock gene expression in both the liver and SCN, whereas, a shift in the LD did not move the phase of the liver clock when the shift was carried out under a fixed RF schedule during the night-time. Conclusions: These results suggest that restricted feeding strongly entrained the expression of circadian clock genes in the liver without the participation of an SCN clock function.",
    author = "Reiko Hara and Keiko Wan and Hisanori Wakamatsu and Reiko Aida and Takahiro Moriya and Masashi Akiyama and Shigenobu Shibata",
    year = "2001",
    doi = "10.1046/j.1365-2443.2001.00419.x",
    language = "English",
    volume = "6",
    pages = "269--278",
    journal = "Genes to Cells",
    issn = "1356-9597",
    publisher = "Wiley-Blackwell",
    number = "3",

    }

    TY - JOUR

    T1 - Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus

    AU - Hara, Reiko

    AU - Wan, Keiko

    AU - Wakamatsu, Hisanori

    AU - Aida, Reiko

    AU - Moriya, Takahiro

    AU - Akiyama, Masashi

    AU - Shibata, Shigenobu

    PY - 2001

    Y1 - 2001

    N2 - Background: There are two main stimuli that entrain the circadian rhythm, the light-dark cycle (LD) and restricted feeding (RF). Light-induced entrainment requires induction of the Per1 and Per2 genes in the suprachiasmatic nucleus (SCN), the locus of a main oscillator. In this experiment, we determined whether RF resets the expression of circadian clock genes in the mouse liver with or without participation of the SCN. Results: Mice were allowed access to food for 4 h during the daytime (7 h advance of feeding time) under LD or constant darkness (DD). The peaks of mPer1, mPer2, D-site-binding protein (Dbp) and cholesterol 7α-hydroxylase (Cyp7A) mRNA in the liver were advanced 6-12 h after 6 days of RF, whereas those in SCN were unaffected. The advance of mPer expression in the liver by RF was still observed in SCN-lesioned mice. A 7 h advance in the LD cycle advanced the peaks of clock gene expression in both the liver and SCN, whereas, a shift in the LD did not move the phase of the liver clock when the shift was carried out under a fixed RF schedule during the night-time. Conclusions: These results suggest that restricted feeding strongly entrained the expression of circadian clock genes in the liver without the participation of an SCN clock function.

    AB - Background: There are two main stimuli that entrain the circadian rhythm, the light-dark cycle (LD) and restricted feeding (RF). Light-induced entrainment requires induction of the Per1 and Per2 genes in the suprachiasmatic nucleus (SCN), the locus of a main oscillator. In this experiment, we determined whether RF resets the expression of circadian clock genes in the mouse liver with or without participation of the SCN. Results: Mice were allowed access to food for 4 h during the daytime (7 h advance of feeding time) under LD or constant darkness (DD). The peaks of mPer1, mPer2, D-site-binding protein (Dbp) and cholesterol 7α-hydroxylase (Cyp7A) mRNA in the liver were advanced 6-12 h after 6 days of RF, whereas those in SCN were unaffected. The advance of mPer expression in the liver by RF was still observed in SCN-lesioned mice. A 7 h advance in the LD cycle advanced the peaks of clock gene expression in both the liver and SCN, whereas, a shift in the LD did not move the phase of the liver clock when the shift was carried out under a fixed RF schedule during the night-time. Conclusions: These results suggest that restricted feeding strongly entrained the expression of circadian clock genes in the liver without the participation of an SCN clock function.

    UR - http://www.scopus.com/inward/record.url?scp=0035059442&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0035059442&partnerID=8YFLogxK

    U2 - 10.1046/j.1365-2443.2001.00419.x

    DO - 10.1046/j.1365-2443.2001.00419.x

    M3 - Article

    VL - 6

    SP - 269

    EP - 278

    JO - Genes to Cells

    JF - Genes to Cells

    SN - 1356-9597

    IS - 3

    ER -