Restricted feeding entrains liver clock without participation of the suprachiasmatic nucleus

Reiko Hara, Keiko Wan, Hisanori Wakamatsu, Reiko Aida, Takahiro Moriya, Masashi Akiyama, Shigenobu Shibata

Research output: Contribution to journalArticle

414 Citations (Scopus)

Abstract

Background: There are two main stimuli that entrain the circadian rhythm, the light-dark cycle (LD) and restricted feeding (RF). Light-induced entrainment requires induction of the Per1 and Per2 genes in the suprachiasmatic nucleus (SCN), the locus of a main oscillator. In this experiment, we determined whether RF resets the expression of circadian clock genes in the mouse liver with or without participation of the SCN. Results: Mice were allowed access to food for 4 h during the daytime (7 h advance of feeding time) under LD or constant darkness (DD). The peaks of mPer1, mPer2, D-site-binding protein (Dbp) and cholesterol 7α-hydroxylase (Cyp7A) mRNA in the liver were advanced 6-12 h after 6 days of RF, whereas those in SCN were unaffected. The advance of mPer expression in the liver by RF was still observed in SCN-lesioned mice. A 7 h advance in the LD cycle advanced the peaks of clock gene expression in both the liver and SCN, whereas, a shift in the LD did not move the phase of the liver clock when the shift was carried out under a fixed RF schedule during the night-time. Conclusions: These results suggest that restricted feeding strongly entrained the expression of circadian clock genes in the liver without the participation of an SCN clock function.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalGenes to Cells
Volume6
Issue number3
DOIs
Publication statusPublished - 2001 Apr 18

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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