Retinotectal transmission in the optic tectum of rainbow trout

Masae Kinoshita, Masahiro Fukaya, Takuro Tojima, Satoshi Kojima, Hironori Ando, Masahiko Watanabe, Akihisa Urano, Etsuro Ito

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Retinotectal transmission has not yet been well characterized at the cellular level in the optic tectum. To address this issue, we used a teleost, the rainbow trout, and characterized periventricular neurons as postsynaptic cells expected to receive the retinotectal inputs to the optic tectum. The somata of periventricular neurons are localized in the upper zone of the stratum periventriculare (SPV), whereas the lower zone of the SPV comprises the cell body layer of radial glial cells. Ca2+ imaging identified functional ionotropic glutamate receptors in periventricular neurons. We also cloned cDNAs encoding the NR1 subunit of N-methyl-D-aspartic acid (NMDA) receptors and the GluR2 subunit of (±)-α-amino-3-hydroxy-5-methyl-isoxazole-4- propionic acid (AMPA) receptors, and detected their mRNAs in periventricular neurons by in situ hybridization. The presence of the receptor subunit proteins was also confirmed in the dendrites of periventricular neurons by immunoblotting and immunohistochemistry. On the other hand, radial glial cells in the lower zone of the SPV did not respond to glutamate applications, and mRNA and immunoreactivities of ionotropic glutamate receptors were not detected in glial cells. The present findings suggest that glutamatergic transmission at synapses between retinotectal afferents and periventricular neurons is mediated by the functional NMDA and AMPA receptors.

Original languageEnglish
Pages (from-to)249-259
Number of pages11
JournalJournal of Comparative Neurology
Volume484
Issue number2
DOIs
Publication statusPublished - 2005 Apr 4
Externally publishedYes

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Superior Colliculi
Oncorhynchus mykiss
Neurons
Ependymoglial Cells
Ionotropic Glutamate Receptors
AMPA Receptors
N-Methylaspartate
Isoxazoles
Afferent Neurons
Messenger RNA
Protein Subunits
Carisoprodol
Dendrites
Immunoblotting
Neuroglia
Synapses
In Situ Hybridization
Glutamic Acid
Complementary DNA
Immunohistochemistry

Keywords

  • AMPA
  • Glutamate receptor
  • NMDA
  • Radial glia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kinoshita, M., Fukaya, M., Tojima, T., Kojima, S., Ando, H., Watanabe, M., ... Ito, E. (2005). Retinotectal transmission in the optic tectum of rainbow trout. Journal of Comparative Neurology, 484(2), 249-259. https://doi.org/10.1002/cne.20473

Retinotectal transmission in the optic tectum of rainbow trout. / Kinoshita, Masae; Fukaya, Masahiro; Tojima, Takuro; Kojima, Satoshi; Ando, Hironori; Watanabe, Masahiko; Urano, Akihisa; Ito, Etsuro.

In: Journal of Comparative Neurology, Vol. 484, No. 2, 04.04.2005, p. 249-259.

Research output: Contribution to journalArticle

Kinoshita, M, Fukaya, M, Tojima, T, Kojima, S, Ando, H, Watanabe, M, Urano, A & Ito, E 2005, 'Retinotectal transmission in the optic tectum of rainbow trout', Journal of Comparative Neurology, vol. 484, no. 2, pp. 249-259. https://doi.org/10.1002/cne.20473
Kinoshita M, Fukaya M, Tojima T, Kojima S, Ando H, Watanabe M et al. Retinotectal transmission in the optic tectum of rainbow trout. Journal of Comparative Neurology. 2005 Apr 4;484(2):249-259. https://doi.org/10.1002/cne.20473
Kinoshita, Masae ; Fukaya, Masahiro ; Tojima, Takuro ; Kojima, Satoshi ; Ando, Hironori ; Watanabe, Masahiko ; Urano, Akihisa ; Ito, Etsuro. / Retinotectal transmission in the optic tectum of rainbow trout. In: Journal of Comparative Neurology. 2005 ; Vol. 484, No. 2. pp. 249-259.
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