Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization

Liming Xu, Hidemitsu Harada, Tamaki Yokohama-Tamaki, Shuichiro Matsumoto, Junzo Tanaka, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological activity of amelogenin on ameloblast differentiation. Recombinant mouse amelogenin (rm-amelogenin) enhanced the expression of endogenous amelogenin mRNA in a cultured dental epithelial cell line (HAT-7), despite a lack of increased amelogenin promoter activity. To solve this discrepancy, we analyzed the effects of rm-amelogenin on the stability of amelogenin mRNA. The half-life of amelogenin mRNA is extremely short, but in the presence of rm-amelogenin its half-life was extended three times longer than the control. Furthermore, we showed the entry of exogenous fluorescein isothiocyanate-conjugated rm-amelogenin into the cytoplasm of HAT-7 cells. It follows from our results that exogenous amelogenin increases amelogenin mRNA levels through stabilization of mRNA in the cytoplasm of HAT-7 cells. Here we speculated that during differentiation, dental epithelial cells utilize a unique mechanism for increasing the production of amelogenin, the reuptake of secreted amelogenin.

Original languageEnglish
Pages (from-to)2257-2262
Number of pages6
JournalJournal of Biological Chemistry
Volume281
Issue number4
DOIs
Publication statusPublished - 2006 Jan 27
Externally publishedYes

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Amelogenin
Tooth
Stabilization
Epithelial Cells
Messenger RNA
Enamels
Dental Enamel
Ameloblasts
Bioactivity
Half-Life
Cytoplasm
Extracellular Matrix Proteins
RNA Stability
Fluorescein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization. / Xu, Liming; Harada, Hidemitsu; Yokohama-Tamaki, Tamaki; Matsumoto, Shuichiro; Tanaka, Junzo; Taniguchi, Akiyoshi.

In: Journal of Biological Chemistry, Vol. 281, No. 4, 27.01.2006, p. 2257-2262.

Research output: Contribution to journalArticle

Xu, Liming ; Harada, Hidemitsu ; Yokohama-Tamaki, Tamaki ; Matsumoto, Shuichiro ; Tanaka, Junzo ; Taniguchi, Akiyoshi. / Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 4. pp. 2257-2262.
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