RFamide-related peptide and messenger ribonucleic acid expression in mammalian testis

Association with the Spermatogenic cycle

Sheng Zhao, Edward Zhu, Christina Yang, George E. Bentley, Kazuyoshi Tsutsui, Lance J. Kriegsfeld

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    RFamide-related peptide (RFRP), the mammalian homolog of avian gonadotropin-inhibitory hormone, has a pronounced suppressive action on the reproductive axis across species. In mammals, RFRP acts directly on GnRH neurons, and likely at the level of the pituitary, to inhibit gonadotropin secretion. In the present study, we examined whether RFRP might act outside of mammalian brain on reproductive tissues directly. Using RT-PCR and in situ hybridization, we found that both RFRP and its receptors [G protein-coupled receptor (GPR) 147 and GPR74] are expressed in the testis of Syrian hamster. These results were confirmed and extended using double-andtriple-label immunohistochemistry. RFRP expression was observed in spermatocytes and in round to early elongated spermatids. Significant expression of RFRP was not seen in Leydig cells. GPR147 protein was observed in myoid cells in all stages of spermatogenesis, pachytene spermatocytes, maturation division spermatocytes, and in round and late elongated spermatids. GPR74 proteins only appeared in late elongated spermatids. Additionally,wefound that RFRP and its receptor mRNA are markedly altered by day length and reproductive condition. These findings highlight a possible novel autocrine and/or paracrine role for RFRP in Syrian hamster testis, potentially contributing to the differentiation of spermatids during spermiogenesis.

    Original languageEnglish
    Pages (from-to)617-627
    Number of pages11
    JournalEndocrinology
    Volume151
    Issue number2
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Testis
    RNA
    Spermatids
    Spermatocytes
    Peptide Receptors
    Mesocricetus
    Spermatogenesis
    Gonadotropins
    Pachytene Stage
    RFamide peptide
    Leydig Cells
    G-Protein-Coupled Receptors
    Gonadotropin-Releasing Hormone
    In Situ Hybridization
    Mammals
    Proteins
    Immunohistochemistry
    Hormones
    Neurons
    Polymerase Chain Reaction

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    RFamide-related peptide and messenger ribonucleic acid expression in mammalian testis : Association with the Spermatogenic cycle. / Zhao, Sheng; Zhu, Edward; Yang, Christina; Bentley, George E.; Tsutsui, Kazuyoshi; Kriegsfeld, Lance J.

    In: Endocrinology, Vol. 151, No. 2, 2010, p. 617-627.

    Research output: Contribution to journalArticle

    Zhao, Sheng ; Zhu, Edward ; Yang, Christina ; Bentley, George E. ; Tsutsui, Kazuyoshi ; Kriegsfeld, Lance J. / RFamide-related peptide and messenger ribonucleic acid expression in mammalian testis : Association with the Spermatogenic cycle. In: Endocrinology. 2010 ; Vol. 151, No. 2. pp. 617-627.
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