RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells

Satoshi Arai, Rókus Kriszt, Kazuki Harada, Liang Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, Shoichi Ishiura, Yu Hua Tseng, Michael Raghunath, Toshiro Ito, Takashi Tsuboi, Tetsuya Kitaguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

Original languageEnglish
Pages (from-to)10873-10878
Number of pages6
JournalAngewandte Chemie - International Edition
Volume57
Issue number34
DOIs
Publication statusPublished - 2018 Aug 20

Keywords

  • ATP
  • brown adipocytes
  • fluorescent probes
  • live cell imaging
  • protein engineering

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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    Arai, S., Kriszt, R., Harada, K., Looi, L. S., Matsuda, S., Wongso, D., Suo, S., Ishiura, S., Tseng, Y. H., Raghunath, M., Ito, T., Tsuboi, T., & Kitaguchi, T. (2018). RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells. Angewandte Chemie - International Edition, 57(34), 10873-10878. https://doi.org/10.1002/anie.201804304