RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells

Satoshi Arai, Rókus Kriszt, Kazuki Harada, Liang Sheng Looi, Shogo Matsuda, Devina Wongso, Satoshi Suo, Shoichi Ishiura, Yu Hua Tseng, Michael Raghunath, Toshiro Ito, Takashi Tsuboi, Tetsuya Kitaguchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

Original languageEnglish
Pages (from-to)10873-10878
Number of pages6
JournalAngewandte Chemie - International Edition
Volume57
Issue number34
DOIs
Publication statusPublished - 2018 Aug 20

Fingerprint

Adenosine Triphosphate
Color
Mitochondria
Mammals
Visualization
Proteins
Molecules
Energy Metabolism

Keywords

  • ATP
  • brown adipocytes
  • fluorescent probes
  • live cell imaging
  • protein engineering

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Arai, S., Kriszt, R., Harada, K., Looi, L. S., Matsuda, S., Wongso, D., ... Kitaguchi, T. (2018). RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells. Angewandte Chemie - International Edition, 57(34), 10873-10878. https://doi.org/10.1002/anie.201804304

RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells. / Arai, Satoshi; Kriszt, Rókus; Harada, Kazuki; Looi, Liang Sheng; Matsuda, Shogo; Wongso, Devina; Suo, Satoshi; Ishiura, Shoichi; Tseng, Yu Hua; Raghunath, Michael; Ito, Toshiro; Tsuboi, Takashi; Kitaguchi, Tetsuya.

In: Angewandte Chemie - International Edition, Vol. 57, No. 34, 20.08.2018, p. 10873-10878.

Research output: Contribution to journalArticle

Arai, S, Kriszt, R, Harada, K, Looi, LS, Matsuda, S, Wongso, D, Suo, S, Ishiura, S, Tseng, YH, Raghunath, M, Ito, T, Tsuboi, T & Kitaguchi, T 2018, 'RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells', Angewandte Chemie - International Edition, vol. 57, no. 34, pp. 10873-10878. https://doi.org/10.1002/anie.201804304
Arai, Satoshi ; Kriszt, Rókus ; Harada, Kazuki ; Looi, Liang Sheng ; Matsuda, Shogo ; Wongso, Devina ; Suo, Satoshi ; Ishiura, Shoichi ; Tseng, Yu Hua ; Raghunath, Michael ; Ito, Toshiro ; Tsuboi, Takashi ; Kitaguchi, Tetsuya. / RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells. In: Angewandte Chemie - International Edition. 2018 ; Vol. 57, No. 34. pp. 10873-10878.
@article{5f6bffa7e00642e39f0c9343bc1657ae,
title = "RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells",
abstract = "Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.",
keywords = "ATP, brown adipocytes, fluorescent probes, live cell imaging, protein engineering",
author = "Satoshi Arai and R{\'o}kus Kriszt and Kazuki Harada and Looi, {Liang Sheng} and Shogo Matsuda and Devina Wongso and Satoshi Suo and Shoichi Ishiura and Tseng, {Yu Hua} and Michael Raghunath and Toshiro Ito and Takashi Tsuboi and Tetsuya Kitaguchi",
year = "2018",
month = "8",
day = "20",
doi = "10.1002/anie.201804304",
language = "English",
volume = "57",
pages = "10873--10878",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "34",

}

TY - JOUR

T1 - RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells

AU - Arai, Satoshi

AU - Kriszt, Rókus

AU - Harada, Kazuki

AU - Looi, Liang Sheng

AU - Matsuda, Shogo

AU - Wongso, Devina

AU - Suo, Satoshi

AU - Ishiura, Shoichi

AU - Tseng, Yu Hua

AU - Raghunath, Michael

AU - Ito, Toshiro

AU - Tsuboi, Takashi

AU - Kitaguchi, Tetsuya

PY - 2018/8/20

Y1 - 2018/8/20

N2 - Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

AB - Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of ATP in single cells is fundamental to our understanding of the mechanisms underlying cellular energy metabolism. However, it has remained challenging to visualize the dynamics of ATP in and between distinct intracellular organelles and its interplay with other signaling molecules. Using single fluorescent proteins, multicolor ATP indicators were developed, enabling the simultaneous visualization of subcellular ATP dynamics in the cytoplasm and mitochondria of cells derived from mammals, plants, and worms. Furthermore, in combination with additional fluorescent indicators, the dynamic interplay of ATP, cAMP, and Ca2+ could be visualized in activated brown adipocyte. This set of indicator tools will facilitate future research into energy metabolism.

KW - ATP

KW - brown adipocytes

KW - fluorescent probes

KW - live cell imaging

KW - protein engineering

UR - http://www.scopus.com/inward/record.url?scp=85050698563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050698563&partnerID=8YFLogxK

U2 - 10.1002/anie.201804304

DO - 10.1002/anie.201804304

M3 - Article

VL - 57

SP - 10873

EP - 10878

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 34

ER -