Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation

Nobuji Maeda, Kazuhiko Imaizumi, Misuzu Sekiya, Takeshi Shiga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effect of fibrinogen and sialic acid content of erythrocytes on the aggregation of erythrocytes was quantitatively examined by using a rheoscope combined with a television image analyzer and a computer. (1) The electrophoretic mobility of erythrocytes was proportional to the sialic acid content of erythrocytes (the surface potential of erythrocytes could be expressed by the sialic acid content). (2) The aggregation of erythrocytes was accelerated by increasing fibrinogen concentration in the medium (due to the increased bridging force among erythrocytes) or by decreasing the sialic acid content (due to the reduction of the electrostatic repulsive force among erythrocytes). (3) An empirical equation expressing the velocity of aggregate formation (ν, in μm2/min) by the concentration of fibrinogen (F, in g/dl) and the sialic acid content (S, in μmol/ml red blood cells), log ν = -0.065 F-1.2S + 2.2 F0.35, was deduced. (4) The contribution of the bridging force of fibrinogen to the erythrocyte aggregation was much greater than that of the electrostatic repulsive force produced by sialic acid on the surface of erythrocytes.

Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalBBA - Biomembranes
Volume776
Issue number1
DOIs
Publication statusPublished - 1984 Sep 19
Externally publishedYes

Fingerprint

N-Acetylneuraminic Acid
Fibrinogen
Agglomeration
Erythrocytes
Erythrocyte Aggregation
Electrostatic force
Static Electricity
Electrophoretic mobility
Television
Blood
Cells

Keywords

  • (Erythrocyte)
  • Cell aggregation
  • Fibrinogen
  • Rheology
  • Rouleau formation
  • Scanning electron microscopy
  • Sialic acid content

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation. / Maeda, Nobuji; Imaizumi, Kazuhiko; Sekiya, Misuzu; Shiga, Takeshi.

In: BBA - Biomembranes, Vol. 776, No. 1, 19.09.1984, p. 151-158.

Research output: Contribution to journalArticle

Maeda, Nobuji ; Imaizumi, Kazuhiko ; Sekiya, Misuzu ; Shiga, Takeshi. / Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation. In: BBA - Biomembranes. 1984 ; Vol. 776, No. 1. pp. 151-158.
@article{32fce2ee775842a3bfb98a0e41eb2e0b,
title = "Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation",
abstract = "The effect of fibrinogen and sialic acid content of erythrocytes on the aggregation of erythrocytes was quantitatively examined by using a rheoscope combined with a television image analyzer and a computer. (1) The electrophoretic mobility of erythrocytes was proportional to the sialic acid content of erythrocytes (the surface potential of erythrocytes could be expressed by the sialic acid content). (2) The aggregation of erythrocytes was accelerated by increasing fibrinogen concentration in the medium (due to the increased bridging force among erythrocytes) or by decreasing the sialic acid content (due to the reduction of the electrostatic repulsive force among erythrocytes). (3) An empirical equation expressing the velocity of aggregate formation (ν, in μm2/min) by the concentration of fibrinogen (F, in g/dl) and the sialic acid content (S, in μmol/ml red blood cells), log ν = -0.065 F-1.2S + 2.2 F0.35, was deduced. (4) The contribution of the bridging force of fibrinogen to the erythrocyte aggregation was much greater than that of the electrostatic repulsive force produced by sialic acid on the surface of erythrocytes.",
keywords = "(Erythrocyte), Cell aggregation, Fibrinogen, Rheology, Rouleau formation, Scanning electron microscopy, Sialic acid content",
author = "Nobuji Maeda and Kazuhiko Imaizumi and Misuzu Sekiya and Takeshi Shiga",
year = "1984",
month = "9",
day = "19",
doi = "10.1016/0005-2736(84)90261-X",
language = "English",
volume = "776",
pages = "151--158",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation

AU - Maeda, Nobuji

AU - Imaizumi, Kazuhiko

AU - Sekiya, Misuzu

AU - Shiga, Takeshi

PY - 1984/9/19

Y1 - 1984/9/19

N2 - The effect of fibrinogen and sialic acid content of erythrocytes on the aggregation of erythrocytes was quantitatively examined by using a rheoscope combined with a television image analyzer and a computer. (1) The electrophoretic mobility of erythrocytes was proportional to the sialic acid content of erythrocytes (the surface potential of erythrocytes could be expressed by the sialic acid content). (2) The aggregation of erythrocytes was accelerated by increasing fibrinogen concentration in the medium (due to the increased bridging force among erythrocytes) or by decreasing the sialic acid content (due to the reduction of the electrostatic repulsive force among erythrocytes). (3) An empirical equation expressing the velocity of aggregate formation (ν, in μm2/min) by the concentration of fibrinogen (F, in g/dl) and the sialic acid content (S, in μmol/ml red blood cells), log ν = -0.065 F-1.2S + 2.2 F0.35, was deduced. (4) The contribution of the bridging force of fibrinogen to the erythrocyte aggregation was much greater than that of the electrostatic repulsive force produced by sialic acid on the surface of erythrocytes.

AB - The effect of fibrinogen and sialic acid content of erythrocytes on the aggregation of erythrocytes was quantitatively examined by using a rheoscope combined with a television image analyzer and a computer. (1) The electrophoretic mobility of erythrocytes was proportional to the sialic acid content of erythrocytes (the surface potential of erythrocytes could be expressed by the sialic acid content). (2) The aggregation of erythrocytes was accelerated by increasing fibrinogen concentration in the medium (due to the increased bridging force among erythrocytes) or by decreasing the sialic acid content (due to the reduction of the electrostatic repulsive force among erythrocytes). (3) An empirical equation expressing the velocity of aggregate formation (ν, in μm2/min) by the concentration of fibrinogen (F, in g/dl) and the sialic acid content (S, in μmol/ml red blood cells), log ν = -0.065 F-1.2S + 2.2 F0.35, was deduced. (4) The contribution of the bridging force of fibrinogen to the erythrocyte aggregation was much greater than that of the electrostatic repulsive force produced by sialic acid on the surface of erythrocytes.

KW - (Erythrocyte)

KW - Cell aggregation

KW - Fibrinogen

KW - Rheology

KW - Rouleau formation

KW - Scanning electron microscopy

KW - Sialic acid content

UR - http://www.scopus.com/inward/record.url?scp=0021193546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021193546&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(84)90261-X

DO - 10.1016/0005-2736(84)90261-X

M3 - Article

VL - 776

SP - 151

EP - 158

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 1

ER -