Rheological properties of hemoglobin vesicles (artificial oxygen carriers) suspended in a series of plasma-substitute solutions

Hiromi Sakai, Atsushi Sato, Shinji Takeoka, Eishun Tsuchida

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Hemoglobin vesicles (HbV) or liposome-encapsulated Hbs are artificial oxygen carriers that have been developed for use as transfusion alternatives. The extremely high concentration of the HbV suspension (solutes, ca. 16 g/dL; volume fraction, ca. 40 vol%) gives it an oxygen-carrying capacity that is comparable to that of blood. The HbV suspension does not possess a colloid osmotic pressure. Therefore, HbV must be suspended in or co-injected with an aqueous solution of a plasma substitute (water-soluble polymer), which might interact with HbV. This article describes our study of the rheological properties of HbV suspended in a series of plasma substitute solutions of various molecular weights: recombinant human serum albumin (rHSA), dextran (DEX), modified fluid gelatin (MFG), and hydroxylethyl starch (HES). The HbV suspended in rHSA was nearly Newtonian. Other polymers - HES, DEX, and MFG - induced HbV flocculation, possibly by depletion interaction, and rendered the suspensions as non-Newtonian with a shear-thinning profile (10 -4-103 s-1). These HbV suspensions showed a high storage modulus (G′) because of the presence of flocculated HbV. However, HbV suspended in rHSA exhibited a very low G′. The viscosities of HbV suspended in DEX, MFG, and high-molecular-weight HES solutions responded quickly to rapid step changes in shear rates of 0.1-100 s-1 and a return to 0.1 s-1, indicating that flocculation is both rapid and reversible. Microscopically, the flow pattern of the flocculated HbV that perfused through microchannels (4.5 μm deep, 7 μm wide, 20 cmH 2O applied pressure) showed no plugging. Furthermore, the time required for passage was simply proportional to the viscosity. Collectively, the HbV suspension viscosity was influenced by the presence of plasma substitutes. The HbV suspension provides a unique opportunity to manipulate rheological properties for various clinical applications in addition to its use as a transfusion alternative.

    Original languageEnglish
    Pages (from-to)8121-8128
    Number of pages8
    JournalLangmuir
    Volume23
    Issue number15
    DOIs
    Publication statusPublished - 2007 Jul 17

    Fingerprint

    Plasma Substitutes
    Hemoglobin
    hemoglobin
    Hemoglobins
    substitutes
    Oxygen
    Plasmas
    oxygen
    Suspensions
    dextrans
    Dextran
    starches
    gelatins
    Dextrans
    Starch
    albumins
    Serum Albumin
    transfusion
    serums
    Flocculation

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

    Cite this

    Rheological properties of hemoglobin vesicles (artificial oxygen carriers) suspended in a series of plasma-substitute solutions. / Sakai, Hiromi; Sato, Atsushi; Takeoka, Shinji; Tsuchida, Eishun.

    In: Langmuir, Vol. 23, No. 15, 17.07.2007, p. 8121-8128.

    Research output: Contribution to journalArticle

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