Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells

Makiko Watanabe, Akiko Hida, Shingo Kitamura, Minori Enomoto, Yosuke Ohsawa, Yasuko Katayose, Kentaro Nozaki, Yoshiya Moriguchi, Sayaka Aritake, Shigekazu Higuchi, Miyuki Tamura, Mie Kato, Kazuo Mishima

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Evaluating individual circadian rhythm traits is crucial for understanding the human biological clock system. The present study reports characterization of physiological and molecular parameters in 13 healthy male subjects under a constant routine condition, where interfering factors were kept to minimum. We measured hormonal secretion levels and examined temporal expression profiles of circadian clock genes in peripheral leukocytes and beard hair follicle cells. All 13 subjects had prominent daily rhythms in melatonin and cortisol secretion. Significant circadian rhythmicity was found for PER1 in 9 subjects, PER2 in 3 subjects, PER3 in all 13 subjects, and BMAL1 in 8 subjects in leukocytes. Additionally, significant circadian rhythmicity was found for PER1 in 5 of 8 subjects tested, PER2 in 2 subjects, PER3 in 6 subjects, and BMAL1 in 3 subjects in beard hair follicle cells. The phase of PER1 and PER3 rhythms in leukocytes correlated significantly with that of physiological rhythms. Our results demonstrate that leukocytes and beard hair follicle cells possess an endogenous circadian clock and suggest that PER1 and PER3 expression would be appropriate biomarkers and hair follicle cells could be a useful tissue source for the evaluation of biological clock traits in individuals.

Original languageEnglish
Pages (from-to)902-907
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume425
Issue number4
DOIs
Publication statusPublished - 2012 Sep 7
Externally publishedYes

Fingerprint

Circadian Clocks
Hair Follicle
Clocks
Leukocytes
Genes
Biological Clocks
Periodicity
Biomarkers
Melatonin
Circadian Rhythm
Hydrocortisone
Healthy Volunteers
Tissue

Keywords

  • Biomarker
  • Human
  • PER1
  • PER3
  • Surrogate tissue source

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells. / Watanabe, Makiko; Hida, Akiko; Kitamura, Shingo; Enomoto, Minori; Ohsawa, Yosuke; Katayose, Yasuko; Nozaki, Kentaro; Moriguchi, Yoshiya; Aritake, Sayaka; Higuchi, Shigekazu; Tamura, Miyuki; Kato, Mie; Mishima, Kazuo.

In: Biochemical and Biophysical Research Communications, Vol. 425, No. 4, 07.09.2012, p. 902-907.

Research output: Contribution to journalArticle

Watanabe, M, Hida, A, Kitamura, S, Enomoto, M, Ohsawa, Y, Katayose, Y, Nozaki, K, Moriguchi, Y, Aritake, S, Higuchi, S, Tamura, M, Kato, M & Mishima, K 2012, 'Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells', Biochemical and Biophysical Research Communications, vol. 425, no. 4, pp. 902-907. https://doi.org/10.1016/j.bbrc.2012.08.008
Watanabe, Makiko ; Hida, Akiko ; Kitamura, Shingo ; Enomoto, Minori ; Ohsawa, Yosuke ; Katayose, Yasuko ; Nozaki, Kentaro ; Moriguchi, Yoshiya ; Aritake, Sayaka ; Higuchi, Shigekazu ; Tamura, Miyuki ; Kato, Mie ; Mishima, Kazuo. / Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 425, No. 4. pp. 902-907.
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