Ribosomal Protein S1 Is not Essential for the trans-translation Machinery

Hao Qi, Yoshihiro Shimizu, Takuya Ueda

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

In eubacteria, ribosome stalling during protein synthesis is rescued by a tmRNA-derived trans-translation system. Because ribosomal protein S1 specifically binds to tmRNA with high affinity, it is considered to be involved in the trans-translation system. However, the role of S1 in trans-translation is still unclear. To study the function of S1 in the trans-translation system, we constructed an S1-free cell-free translation system. We found that trans-translation proceeded even in the absence of S1. Addition of S1 into the S1-free system did not affect trans-translation efficiency. These results suggest that S1 does not play a role in the trans-translation machinery.

Original languageEnglish
Pages (from-to)845-852
Number of pages8
JournalJournal of Molecular Biology
Volume368
Issue number3
DOIs
Publication statusPublished - 2007 May 4
Externally publishedYes

Keywords

  • ribosomal protein S1
  • ribosome
  • tmRNA
  • trans-translation
  • translation

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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