Right-handed rotation of an actin filament in an in vitro motile system

Takayuki Nishizaka, Toshiki Yagi, Yuichiro Tanaka, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    93 Citations (Scopus)

    Abstract

    MUSCLE contraction occurs by mutual sliding between thick (myosin) and thin (actin) filaments1,2. But the physical and chemical properties of the sliding force are not clear; even the precise direction of sliding force generated at each cross-bridge is not known. We report here the use of a recently developed in vitro motile assay system3-5 to show supercoiling of an actin filament in which the front part of the filament was fixed to a glass surface through cross-linked heavy-meromyosin and the rear part was able to slide on a track of heavy-meromyosin. A left-handed single turn of superhelix formed just before supercoiling, suggesting that the sliding force has a right-handed torque component that induces the right-handed rotation of an actin filament around its long axis. The presence of the torque component in the sliding force will explain several properties of the contractile system of muscle.

    Original languageEnglish
    Pages (from-to)269-271
    Number of pages3
    JournalNature
    Volume361
    Issue number6409
    Publication statusPublished - 1993 Jan 21

    Fingerprint

    Myosin Subfragments
    Torque
    Actin Cytoskeleton
    Myosins
    Glass
    Actins
    Muscles
    In Vitro Techniques
    Direction compound

    ASJC Scopus subject areas

    • General

    Cite this

    Nishizaka, T., Yagi, T., Tanaka, Y., & Ishiwata, S. (1993). Right-handed rotation of an actin filament in an in vitro motile system. Nature, 361(6409), 269-271.

    Right-handed rotation of an actin filament in an in vitro motile system. / Nishizaka, Takayuki; Yagi, Toshiki; Tanaka, Yuichiro; Ishiwata, Shin'ichi.

    In: Nature, Vol. 361, No. 6409, 21.01.1993, p. 269-271.

    Research output: Contribution to journalArticle

    Nishizaka, T, Yagi, T, Tanaka, Y & Ishiwata, S 1993, 'Right-handed rotation of an actin filament in an in vitro motile system', Nature, vol. 361, no. 6409, pp. 269-271.
    Nishizaka T, Yagi T, Tanaka Y, Ishiwata S. Right-handed rotation of an actin filament in an in vitro motile system. Nature. 1993 Jan 21;361(6409):269-271.
    Nishizaka, Takayuki ; Yagi, Toshiki ; Tanaka, Yuichiro ; Ishiwata, Shin'ichi. / Right-handed rotation of an actin filament in an in vitro motile system. In: Nature. 1993 ; Vol. 361, No. 6409. pp. 269-271.
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