RNA-binding protein Musashi2: Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS

Shinichi Sakakibara, Yuki Nakamura, Hitoshi Satoh, Hideyuki Okano

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Musashi1 (Msi1) is a mammalian neural RNA-binding protein highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic and post-natal CNS development. Here, we identified Musashi2 (Msi2), a novel mammalian RNA-binding protein that exhibits high sequence similarity to Msi1. The Msi2 transcript appeared to be distributed ubiquitously in a wide variety of tissues, consistent with the mRNA distribution of its Xenopus homolog, xrp1. However, the present study revealed cell type-specific and developmentally regulated expression of Msi2 in the mammalian CNS. Interestingly, Msi2 was expressed prominently in precursor cells in the ventricular zone and subventricular zone with the same pattern as Msi1 throughout CNS development. In the postnatal and adult CNS, this concurrent expression of Msi2 and Msi1 was seen in cells of the astrocyte lineage, including ependymal cells, a possible source for postnatal CNS stem cells. During neurogenesis, the expression of both Msi2 and Msi1 was lost in most postmitotic neurons, whereas Msi2 expression persisted in a subset of neuronal lineage cells, such as parvalbumin-containing GABA neurons in the neocortex and neurons in several nuclei of the basal ganglia. Msi2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages. Furthermore, in vitro studies showed that Msi2 and Msi1 have similar RNA-binding specificity. These two RNA-binding proteins may exert common functions in neural precursor cells by regulating gene expression at the post-transcriptional level.

Original languageEnglish
Pages (from-to)8091-8107
Number of pages17
JournalJournal of Neuroscience
Volume21
Issue number20
Publication statusPublished - 2001 Oct 15
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Neurons
GABAergic Neurons
Parvalbumins
Lateral Ventricles
Neocortex
Neurogenesis
Cell Lineage
Xenopus
Basal Ganglia
Neuroglia
Astrocytes
Stem Cells
Maintenance
RNA
Gene Expression
Messenger RNA

Keywords

  • Astrocyte
  • CNS development
  • CNS stem cell
  • GABAergic neuron
  • Musashi1
  • Musashi2
  • Neural precursor cells
  • Parvalbumin
  • RNA-binding protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

RNA-binding protein Musashi2 : Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. / Sakakibara, Shinichi; Nakamura, Yuki; Satoh, Hitoshi; Okano, Hideyuki.

In: Journal of Neuroscience, Vol. 21, No. 20, 15.10.2001, p. 8091-8107.

Research output: Contribution to journalArticle

@article{19db523d2ddc462686829ec4e6139530,
title = "RNA-binding protein Musashi2: Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS",
abstract = "Musashi1 (Msi1) is a mammalian neural RNA-binding protein highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic and post-natal CNS development. Here, we identified Musashi2 (Msi2), a novel mammalian RNA-binding protein that exhibits high sequence similarity to Msi1. The Msi2 transcript appeared to be distributed ubiquitously in a wide variety of tissues, consistent with the mRNA distribution of its Xenopus homolog, xrp1. However, the present study revealed cell type-specific and developmentally regulated expression of Msi2 in the mammalian CNS. Interestingly, Msi2 was expressed prominently in precursor cells in the ventricular zone and subventricular zone with the same pattern as Msi1 throughout CNS development. In the postnatal and adult CNS, this concurrent expression of Msi2 and Msi1 was seen in cells of the astrocyte lineage, including ependymal cells, a possible source for postnatal CNS stem cells. During neurogenesis, the expression of both Msi2 and Msi1 was lost in most postmitotic neurons, whereas Msi2 expression persisted in a subset of neuronal lineage cells, such as parvalbumin-containing GABA neurons in the neocortex and neurons in several nuclei of the basal ganglia. Msi2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages. Furthermore, in vitro studies showed that Msi2 and Msi1 have similar RNA-binding specificity. These two RNA-binding proteins may exert common functions in neural precursor cells by regulating gene expression at the post-transcriptional level.",
keywords = "Astrocyte, CNS development, CNS stem cell, GABAergic neuron, Musashi1, Musashi2, Neural precursor cells, Parvalbumin, RNA-binding protein",
author = "Shinichi Sakakibara and Yuki Nakamura and Hitoshi Satoh and Hideyuki Okano",
year = "2001",
month = "10",
day = "15",
language = "English",
volume = "21",
pages = "8091--8107",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "20",

}

TY - JOUR

T1 - RNA-binding protein Musashi2

T2 - Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS

AU - Sakakibara, Shinichi

AU - Nakamura, Yuki

AU - Satoh, Hitoshi

AU - Okano, Hideyuki

PY - 2001/10/15

Y1 - 2001/10/15

N2 - Musashi1 (Msi1) is a mammalian neural RNA-binding protein highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic and post-natal CNS development. Here, we identified Musashi2 (Msi2), a novel mammalian RNA-binding protein that exhibits high sequence similarity to Msi1. The Msi2 transcript appeared to be distributed ubiquitously in a wide variety of tissues, consistent with the mRNA distribution of its Xenopus homolog, xrp1. However, the present study revealed cell type-specific and developmentally regulated expression of Msi2 in the mammalian CNS. Interestingly, Msi2 was expressed prominently in precursor cells in the ventricular zone and subventricular zone with the same pattern as Msi1 throughout CNS development. In the postnatal and adult CNS, this concurrent expression of Msi2 and Msi1 was seen in cells of the astrocyte lineage, including ependymal cells, a possible source for postnatal CNS stem cells. During neurogenesis, the expression of both Msi2 and Msi1 was lost in most postmitotic neurons, whereas Msi2 expression persisted in a subset of neuronal lineage cells, such as parvalbumin-containing GABA neurons in the neocortex and neurons in several nuclei of the basal ganglia. Msi2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages. Furthermore, in vitro studies showed that Msi2 and Msi1 have similar RNA-binding specificity. These two RNA-binding proteins may exert common functions in neural precursor cells by regulating gene expression at the post-transcriptional level.

AB - Musashi1 (Msi1) is a mammalian neural RNA-binding protein highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic and post-natal CNS development. Here, we identified Musashi2 (Msi2), a novel mammalian RNA-binding protein that exhibits high sequence similarity to Msi1. The Msi2 transcript appeared to be distributed ubiquitously in a wide variety of tissues, consistent with the mRNA distribution of its Xenopus homolog, xrp1. However, the present study revealed cell type-specific and developmentally regulated expression of Msi2 in the mammalian CNS. Interestingly, Msi2 was expressed prominently in precursor cells in the ventricular zone and subventricular zone with the same pattern as Msi1 throughout CNS development. In the postnatal and adult CNS, this concurrent expression of Msi2 and Msi1 was seen in cells of the astrocyte lineage, including ependymal cells, a possible source for postnatal CNS stem cells. During neurogenesis, the expression of both Msi2 and Msi1 was lost in most postmitotic neurons, whereas Msi2 expression persisted in a subset of neuronal lineage cells, such as parvalbumin-containing GABA neurons in the neocortex and neurons in several nuclei of the basal ganglia. Msi2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages. Furthermore, in vitro studies showed that Msi2 and Msi1 have similar RNA-binding specificity. These two RNA-binding proteins may exert common functions in neural precursor cells by regulating gene expression at the post-transcriptional level.

KW - Astrocyte

KW - CNS development

KW - CNS stem cell

KW - GABAergic neuron

KW - Musashi1

KW - Musashi2

KW - Neural precursor cells

KW - Parvalbumin

KW - RNA-binding protein

UR - http://www.scopus.com/inward/record.url?scp=0035887898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035887898&partnerID=8YFLogxK

M3 - Article

C2 - 11588182

AN - SCOPUS:0035887898

VL - 21

SP - 8091

EP - 8107

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 20

ER -