RNA interference of gonadotropin-inhibitory hormone gene induces aggressive and sexual behaviors in birds

Takayoshi Ubuka, Takanobu Mizuno, Yujiro Fukuda, George E. Bentley, John C. Wingfield, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Gonadotropin-inhibitory hormone (GnIH) was originally identified in the Japanese quail as a hypothalamic neuropeptide inhibitor of pituitary gonadotropin synthesis and release. GnIH neuronal fibers not only terminate in the median eminence to control anterior pituitary function but also extend widely in the brain, suggesting multiple roles in the regulation of behavior. To identify the role of GnIH neurons in the regulation of behavior, we tested the effect of RNA interference (RNAi) of the GnIH gene on aggressive and sexual behaviors of white-crowned sparrows and Japanese quail. Administration of small interfering RNA against GnIH precursor mRNA (GnIH siRNA) into the third ventricle of white-crowned sparrows reduced resting time, spontaneous production of complex vocalizations, and stimulated brief agonistic vocalizations. These behaviors resembled those of breeding birds during territorial defense. Central administration of GnIH siRNA induced aggressive and sexual behaviors and GnIH administration suppressed GnIH RNAi induced aggressive and sexual behaviors in the male quail. In summary, GnIH may function as a central nervous system suppressor of social interaction, thus playing an important role in the control of reproductive behavior, general aggression and territorial defense.

    Original languageEnglish
    Pages (from-to)179-186
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume181
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan 5

    Fingerprint

    sexual behavior
    gonadotropins
    RNA Interference
    RNA interference
    Gonadotropins
    Sexual Behavior
    Birds
    aggression
    hormones
    Hormones
    birds
    Genes
    genes
    small interfering RNA
    Small Interfering RNA
    Sparrows
    Coturnix
    Passeriformes
    vocalization
    pituitary gonadotropins

    Keywords

    • Aggressive behavior
    • Birds
    • Gonadotropin-inhibitory hormone gene
    • RNA interference
    • Sexual behavior
    • Social interaction

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    RNA interference of gonadotropin-inhibitory hormone gene induces aggressive and sexual behaviors in birds. / Ubuka, Takayoshi; Mizuno, Takanobu; Fukuda, Yujiro; Bentley, George E.; Wingfield, John C.; Tsutsui, Kazuyoshi.

    In: General and Comparative Endocrinology, Vol. 181, No. 1, 05.01.2013, p. 179-186.

    Research output: Contribution to journalArticle

    Ubuka, Takayoshi ; Mizuno, Takanobu ; Fukuda, Yujiro ; Bentley, George E. ; Wingfield, John C. ; Tsutsui, Kazuyoshi. / RNA interference of gonadotropin-inhibitory hormone gene induces aggressive and sexual behaviors in birds. In: General and Comparative Endocrinology. 2013 ; Vol. 181, No. 1. pp. 179-186.
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