RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Tatsuhiko Hoshino, Kazuhiro Furukawa, Satoshi Tsuneda, Yuhei Inamori

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

    Original languageEnglish
    Pages (from-to)406-410
    Number of pages5
    JournalJournal of Microbiological Methods
    Volume69
    Issue number2
    DOIs
    Publication statusPublished - 2007 May

    Fingerprint

    RNA
    Oligonucleotide Probes
    Glass
    Staining and Labeling

    Keywords

    • Hybridization
    • Relative abundance
    • RNA microarray

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Microbiology

    Cite this

    RNA microarray for estimating relative abundance of 16S rRNA in microbial communities. / Hoshino, Tatsuhiko; Furukawa, Kazuhiro; Tsuneda, Satoshi; Inamori, Yuhei.

    In: Journal of Microbiological Methods, Vol. 69, No. 2, 05.2007, p. 406-410.

    Research output: Contribution to journalArticle

    Hoshino, Tatsuhiko ; Furukawa, Kazuhiro ; Tsuneda, Satoshi ; Inamori, Yuhei. / RNA microarray for estimating relative abundance of 16S rRNA in microbial communities. In: Journal of Microbiological Methods. 2007 ; Vol. 69, No. 2. pp. 406-410.
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