RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Tatsuhiko Hoshino, Kazuhiro Furukawa, Satoshi Tsuneda, Yuhei Inamori

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

Original languageEnglish
Pages (from-to)406-410
Number of pages5
JournalJournal of Microbiological Methods
Volume69
Issue number2
DOIs
Publication statusPublished - 2007 May 1

Keywords

  • Hybridization
  • RNA microarray
  • Relative abundance

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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