RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Tatsuhiko Hoshino, Kazuhiro Furukawa, Satoshi Tsuneda, Yuhei Inamori

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    5 Citations (Scopus)

    Abstract

    We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

    Original languageEnglish
    Pages (from-to)406-410
    Number of pages5
    JournalJournal of Microbiological Methods
    Volume69
    Issue number2
    DOIs
    Publication statusPublished - 2007 May

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    Keywords

    • Hybridization
    • Relative abundance
    • RNA microarray

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Microbiology

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