RNA polymerase III dependence of the human L1 promoter and possible participation of the RNA polymerase II factor YY1 in the RNA polymerase III transcription system

Kouichi Kurose, Kikumi Hata, Masahira Hattori, Yoshiyuki Sakaki

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41 Citations (Scopus)

Abstract

From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed through an internal promoter. And the L1 Internal promoter, unlike other internal promoters, is thought to be RNA polymerase II (pol II) dependent, because the L1 transcript has a large size (̃6 kb), protein coding capacity and a 3′ terminal polyadenylation signal followed by a poly(A) tall, and also because transcription from the promoter of Drosophila L1-like element Jockey was highly sensitive to α-amanitin. However, our in vitro transcription study reveals that transcription from the human L1 promoter is highly sensitive to tagetitoxin, a selective inhibitor of RNA polymerase III (pol III), but insensitive to 1 μg/ml of α-amanitin, indicating that the human L1 promoter is pol III-dependent. The pol III dependence is further supported by our observation that L1 and pol III-dependent tRNA gene promoters share a common nuclear factor YY1. There is evidence that YY1 is also a pol II transcription factor. We thus propose that YY1 is a possible member of the pol III transcription system.

Original languageEnglish
Pages (from-to)3704-3709
Number of pages6
JournalNucleic Acids Research
Volume23
Issue number18
DOIs
Publication statusPublished - 1995 Sep 25
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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