RNA processing bodies, peroxisomes, golgi bodies, mitochondria, and endoplasmic reticulum tubule junctions frequently pause at cortical microtubules

Takahiro Hamada, Motoki Tominaga, Takashi Fukaya, Masayoshi Nakamura, Akihiko Nakano, Yuichiro Watanabe, Takashi Hashimoto, Tobias I. Baskin

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Organelle motility, essential for cellular function, is driven by the cytoskeleton. In plants, actin filaments sustain the long-distance transport of many types of organelles, and microtubules typically fine-tune the motile behavior. In shoot epidermal cells of Arabidopsis thaliana seedlings, we show here that a type of RNA granule, the RNA processing body (P-body), is transported by actin filaments and pauses at cortical microtubules. Interestingly, removal of microtubules does not change the frequency of P-body pausing. Similarly, we show that Golgi bodies, peroxisomes, and mitochondria all pause at microtubules, and again the frequency of pauses is not appreciably changed after microtubules are depolymerized. To understand the basis for pausing, we examined the endoplasmic reticulum (ER), whose overall architecture depends on actin filaments. By the dual observation of ER and microtubules, we find that stable junctions of tubular ER occur mainly at microtubules. Removal of microtubules reduces the number of stable ER tubule junctions, but those remaining are maintained without microtubules. The results indicate that pausing on microtubules is a common attribute of motile organelles but that microtubules are not required for pausing. We suggest that pausing on microtubules facilitates interactions between the ER and otherwise translocating organelles in the cell cortex.

Original languageEnglish
Pages (from-to)699-708
Number of pages10
JournalPlant and Cell Physiology
Volume53
Issue number4
DOIs
Publication statusPublished - 2012 Apr
Externally publishedYes

Fingerprint

Peroxisomes
peroxisomes
Golgi apparatus
Microtubules
Endoplasmic Reticulum
endoplasmic reticulum
microtubules
Mitochondria
mitochondria
RNA
Organelles
organelles
microfilaments
Actin Cytoskeleton
cytoskeleton
Cytoskeleton
Seedlings
Arabidopsis
granules
cortex

Keywords

  • ER tubule junction
  • Golgi body
  • Microtubule
  • Mitochondrion
  • Peroxisome
  • Processing body

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

RNA processing bodies, peroxisomes, golgi bodies, mitochondria, and endoplasmic reticulum tubule junctions frequently pause at cortical microtubules. / Hamada, Takahiro; Tominaga, Motoki; Fukaya, Takashi; Nakamura, Masayoshi; Nakano, Akihiko; Watanabe, Yuichiro; Hashimoto, Takashi; Baskin, Tobias I.

In: Plant and Cell Physiology, Vol. 53, No. 4, 04.2012, p. 699-708.

Research output: Contribution to journalArticle

Hamada, Takahiro ; Tominaga, Motoki ; Fukaya, Takashi ; Nakamura, Masayoshi ; Nakano, Akihiko ; Watanabe, Yuichiro ; Hashimoto, Takashi ; Baskin, Tobias I. / RNA processing bodies, peroxisomes, golgi bodies, mitochondria, and endoplasmic reticulum tubule junctions frequently pause at cortical microtubules. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 4. pp. 699-708.
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