RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance

Yurika Tachibana, Toshihiro Sakurai, Hanako Bai, Kunio Shiota, Yasuo Nambo, Kentaro Nagaoka, Kazuhiko Imakawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Extensive studies have been conducted to characterize the unique phenomena of equine pregnancy. Most studies have focused on embryo transmigration when the embryo is covered with a mucin-like glycoprotein capsule and on the characterization of the chorionic girdle and chorionic gonadotropin (CG) secretion. However, the events preceding and following capsule disappearance have not been well studied. In this study, the mRNA expression in conceptus membranes at days 19, 21, and 25 (day 05day of ovulation) was analyzed by RNA-seq (SOLiD3), and transcript levels on these three days and day 13 were confirmed by real-time PCR. Of the 26,416 equine genes registered, 20,436 transcripts were aligned to sequences in the Ensembl database, from which 4,625 transcripts were registered in both Ensembl and the KEGG pathway. Each of the 4,625 transcripts was examined through KEGG pathway analysis, and 12 transcripts of integrins (ITGs) and collagens (COLs) were confirmed through real-time PCR. Our data indicated that extracellular matrix (ECM)-related mRNAs were highly expressed in day 19, 21, and 25 conceptus membranes. In combination with previous results, which confirmed a lack of laminin and fibronectin transcript expression in the endometrium, these observations suggest that in contrast to attachment through focal adhesion, conceptus chorionic membrane ECMs function as a scaffold-like structure to possibly maintain the shape of the conceptus and a separation between chorionic membranes and the uterine luminal epithelium.

Original languageEnglish
Article numbere114414
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - 2014 Dec 1
Externally publishedYes

Fingerprint

conceptus
Horses
Capsules
embryo (animal)
Embryonic Structures
RNA
Membranes
horses
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
chorionic gonadotropins
Military electronic countermeasures
Messenger RNA
laminin
Focal Adhesions
fibronectins
mucins
endometrium
integrins
Laminin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tachibana, Y., Sakurai, T., Bai, H., Shiota, K., Nambo, Y., Nagaoka, K., & Imakawa, K. (2014). RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance. PLoS One, 9(12), [e114414]. https://doi.org/10.1371/journal.pone.0114414

RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance. / Tachibana, Yurika; Sakurai, Toshihiro; Bai, Hanako; Shiota, Kunio; Nambo, Yasuo; Nagaoka, Kentaro; Imakawa, Kazuhiko.

In: PLoS One, Vol. 9, No. 12, e114414, 01.12.2014.

Research output: Contribution to journalArticle

Tachibana, Y, Sakurai, T, Bai, H, Shiota, K, Nambo, Y, Nagaoka, K & Imakawa, K 2014, 'RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance', PLoS One, vol. 9, no. 12, e114414. https://doi.org/10.1371/journal.pone.0114414
Tachibana, Yurika ; Sakurai, Toshihiro ; Bai, Hanako ; Shiota, Kunio ; Nambo, Yasuo ; Nagaoka, Kentaro ; Imakawa, Kazuhiko. / RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance. In: PLoS One. 2014 ; Vol. 9, No. 12.
@article{499192bc59b3459fb9e2bbb3b7cbeac8,
title = "RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance",
abstract = "Extensive studies have been conducted to characterize the unique phenomena of equine pregnancy. Most studies have focused on embryo transmigration when the embryo is covered with a mucin-like glycoprotein capsule and on the characterization of the chorionic girdle and chorionic gonadotropin (CG) secretion. However, the events preceding and following capsule disappearance have not been well studied. In this study, the mRNA expression in conceptus membranes at days 19, 21, and 25 (day 05day of ovulation) was analyzed by RNA-seq (SOLiD3), and transcript levels on these three days and day 13 were confirmed by real-time PCR. Of the 26,416 equine genes registered, 20,436 transcripts were aligned to sequences in the Ensembl database, from which 4,625 transcripts were registered in both Ensembl and the KEGG pathway. Each of the 4,625 transcripts was examined through KEGG pathway analysis, and 12 transcripts of integrins (ITGs) and collagens (COLs) were confirmed through real-time PCR. Our data indicated that extracellular matrix (ECM)-related mRNAs were highly expressed in day 19, 21, and 25 conceptus membranes. In combination with previous results, which confirmed a lack of laminin and fibronectin transcript expression in the endometrium, these observations suggest that in contrast to attachment through focal adhesion, conceptus chorionic membrane ECMs function as a scaffold-like structure to possibly maintain the shape of the conceptus and a separation between chorionic membranes and the uterine luminal epithelium.",
author = "Yurika Tachibana and Toshihiro Sakurai and Hanako Bai and Kunio Shiota and Yasuo Nambo and Kentaro Nagaoka and Kazuhiko Imakawa",
year = "2014",
month = "12",
day = "1",
doi = "10.1371/journal.pone.0114414",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - RNA-Seq Analysis of Equine Conceptus Transcripts during Embryo Fixation and Capsule Disappearance

AU - Tachibana, Yurika

AU - Sakurai, Toshihiro

AU - Bai, Hanako

AU - Shiota, Kunio

AU - Nambo, Yasuo

AU - Nagaoka, Kentaro

AU - Imakawa, Kazuhiko

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Extensive studies have been conducted to characterize the unique phenomena of equine pregnancy. Most studies have focused on embryo transmigration when the embryo is covered with a mucin-like glycoprotein capsule and on the characterization of the chorionic girdle and chorionic gonadotropin (CG) secretion. However, the events preceding and following capsule disappearance have not been well studied. In this study, the mRNA expression in conceptus membranes at days 19, 21, and 25 (day 05day of ovulation) was analyzed by RNA-seq (SOLiD3), and transcript levels on these three days and day 13 were confirmed by real-time PCR. Of the 26,416 equine genes registered, 20,436 transcripts were aligned to sequences in the Ensembl database, from which 4,625 transcripts were registered in both Ensembl and the KEGG pathway. Each of the 4,625 transcripts was examined through KEGG pathway analysis, and 12 transcripts of integrins (ITGs) and collagens (COLs) were confirmed through real-time PCR. Our data indicated that extracellular matrix (ECM)-related mRNAs were highly expressed in day 19, 21, and 25 conceptus membranes. In combination with previous results, which confirmed a lack of laminin and fibronectin transcript expression in the endometrium, these observations suggest that in contrast to attachment through focal adhesion, conceptus chorionic membrane ECMs function as a scaffold-like structure to possibly maintain the shape of the conceptus and a separation between chorionic membranes and the uterine luminal epithelium.

AB - Extensive studies have been conducted to characterize the unique phenomena of equine pregnancy. Most studies have focused on embryo transmigration when the embryo is covered with a mucin-like glycoprotein capsule and on the characterization of the chorionic girdle and chorionic gonadotropin (CG) secretion. However, the events preceding and following capsule disappearance have not been well studied. In this study, the mRNA expression in conceptus membranes at days 19, 21, and 25 (day 05day of ovulation) was analyzed by RNA-seq (SOLiD3), and transcript levels on these three days and day 13 were confirmed by real-time PCR. Of the 26,416 equine genes registered, 20,436 transcripts were aligned to sequences in the Ensembl database, from which 4,625 transcripts were registered in both Ensembl and the KEGG pathway. Each of the 4,625 transcripts was examined through KEGG pathway analysis, and 12 transcripts of integrins (ITGs) and collagens (COLs) were confirmed through real-time PCR. Our data indicated that extracellular matrix (ECM)-related mRNAs were highly expressed in day 19, 21, and 25 conceptus membranes. In combination with previous results, which confirmed a lack of laminin and fibronectin transcript expression in the endometrium, these observations suggest that in contrast to attachment through focal adhesion, conceptus chorionic membrane ECMs function as a scaffold-like structure to possibly maintain the shape of the conceptus and a separation between chorionic membranes and the uterine luminal epithelium.

UR - http://www.scopus.com/inward/record.url?scp=84960128519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960128519&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0114414

DO - 10.1371/journal.pone.0114414

M3 - Article

C2 - 25514169

AN - SCOPUS:84960128519

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e114414

ER -