RNase P as hyperprocessing enzyme: A model for formation of a biologically functional tRNA fragment

Yo Kikuchi

Research output: Contribution to journalArticle

Abstract

Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalMolecular Biology Reports: An International Journal on Molecular and Cellular Biology
Volume22
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Ribonuclease P
Transfer RNA
Drosophila
RNA
Enzymes
RNA, Transfer, Met
Catalytic RNA
Retroviridae
Methionine
Reverse Transcription
Catalytic Domain
Nucleotides

Keywords

  • Drosophila
  • hyperprocessing
  • primer
  • retrotransposon copia
  • reverse transcription
  • RNA processing

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

@article{a33f8b518a1a4039ae1bd0e429c41820,
title = "RNase P as hyperprocessing enzyme: A model for formation of a biologically functional tRNA fragment",
abstract = "Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure",
keywords = "Drosophila, hyperprocessing, primer, retrotransposon copia, reverse transcription, RNA processing",
author = "Yo Kikuchi",
year = "1995",
doi = "10.1007/BF00988724",
language = "English",
volume = "22",
pages = "171--175",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - RNase P as hyperprocessing enzyme

T2 - A model for formation of a biologically functional tRNA fragment

AU - Kikuchi, Yo

PY - 1995

Y1 - 1995

N2 - Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure

AB - Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure

KW - Drosophila

KW - hyperprocessing

KW - primer

KW - retrotransposon copia

KW - reverse transcription

KW - RNA processing

UR - http://www.scopus.com/inward/record.url?scp=84951607836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951607836&partnerID=8YFLogxK

U2 - 10.1007/BF00988724

DO - 10.1007/BF00988724

M3 - Article

VL - 22

SP - 171

EP - 175

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 2

ER -