RNase P as hyperprocessing enzyme: A model for formation of a biologically functional tRNA fragment

Yo Kikuchi

Research output: Contribution to journalArticle


Hyperprocessing is defined as a further processing of mature RNA that produces another functional RNA. Hyperprocessing occurs in Drosophila cells. In the transposon copia-related retrovirus-like particles of Drosophila, a 39-nucleotide-long fragment from the 5′-region of Drosophila initiator methionine tRNA is used as the primer for copia minus-strand reverse transcription. This primer tRNA fragment is thought to be produced by cleavage within the mature tRNA sequence. We found that the catalytic RNA subunit of RNase P catalyzes this hyperprocessing in vitro and that this cleavage is dependent of the occurrence of an altered conformation of the tRNA substrate. In this review, I will summarize our work from the finding of the functional RNA fragment to the finding of a dynamic tRNA structure

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalMolecular Biology Reports: An International Journal on Molecular and Cellular Biology
Issue number2
Publication statusPublished - 1995
Externally publishedYes



  • Drosophila
  • hyperprocessing
  • primer
  • retrotransposon copia
  • reverse transcription
  • RNA processing

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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