Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii

Ayumi Minoda, Norihiro Sato, Hisayoshi Nozaki, Katsuhiko Okada, Haruko Takahashi, Kintake Sonoike, Mikio Tsuzuki

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The physiological role of sulfoquinovosyl diacylglycerol (SQDG) in photosynthesis was investigated with a SQDG defective mutant (hf-2) of Chlamydomonas reinhardtii that did not have any detectable amount of SQDG. The mutant showed a lower rate of photosystem II (PSII) activity by ≈ 40% and also a lower growth rate than those of the wild-type. Results of genetical analysis of hf-2 strongly suggest that the SQDG defect and the lowered PSII activity are due to a single gene mutation. The supplementation of SQDG to hf-2 cells restored the lowered PSII activity to the same level as that of wild-type cells, and also enabled the mutant to grow even in the presence of 135 nM 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Moreover, the incubation of isolated thylakoid membranes of hf-2 with SQDG raised the lowered PSII activity. Chemical modifications of SQDG impaired the recovery of PSII activity. The results suggest that SQDG is indispensable for PSII activity in Chlamydomonas by maintaining PSII complexes in their proper state.

Original languageEnglish
Pages (from-to)2353-2358
Number of pages6
JournalEuropean Journal of Biochemistry
Volume269
Issue number9
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Chlamydomonas reinhardtii
Photosystem II Protein Complex
Diglycerides
Maintenance
Diuron
Chlamydomonas
Thylakoids
Photosynthesis
Chemical modification
Genes
Membranes
Recovery
Defects
Mutation

Keywords

  • Chlamydomonas
  • Glycolipid
  • Photosystem II
  • Sulfoquinovosyl diacylglycerol
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii. / Minoda, Ayumi; Sato, Norihiro; Nozaki, Hisayoshi; Okada, Katsuhiko; Takahashi, Haruko; Sonoike, Kintake; Tsuzuki, Mikio.

In: European Journal of Biochemistry, Vol. 269, No. 9, 2002, p. 2353-2358.

Research output: Contribution to journalArticle

Minoda, Ayumi ; Sato, Norihiro ; Nozaki, Hisayoshi ; Okada, Katsuhiko ; Takahashi, Haruko ; Sonoike, Kintake ; Tsuzuki, Mikio. / Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii. In: European Journal of Biochemistry. 2002 ; Vol. 269, No. 9. pp. 2353-2358.
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