Role of the thrombopoietin (TPO)/Mpl system: c-Mpl-like molecule/TPO signaling enhances early hematopoiesis in Xenopus laevis

Minoru Kakeda, Jun Ichi Kyuno, Takashi Kato, Mitsuo Nishikawa, Makoto Asashima

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Multiple organs are induced in the primitive embryonic ectoderm excised from blastula stage Xenopus laevis embryos, under the strict control of mesoderm inducing factors. This in vitro system is useful for exploring the mechanisms of development. In this study, the function of thrombopoietin (TPO)/c-Mpl signaling in the development of hematopoietic cells was investigated. An optimal hematopoietic cell induction system was established to evaluate the influence of growth factors on hematopoiesis. It was found that exogenous TPO enhanced hematopoiesis in explants induced by activin and bone morphogenetic protein (BMP)-4 and increased the number of both erythrocytes and leukocytes in a dose-dependent manner. Addition of anti-c-Mpl antibody completely inhibited the expansion of hematopoietic cells stimulated by TPO, and the antibody specifically recognized blood-like cells. These results demonstrate that TPO acts on hematopoietic progenitors induced in explants and the c-Mpl-like molecule in Xenopus mediates the cellular function of TPO. We also found that forced expression of TPO in embryos promoted hematopoiesis in the ventral blood island and the dorsal-lateral plate mesoderm. These results suggest that hematopoietic stem and progenitor cells are regulated by TPO/c-Mpl signaling from when they appear in their ontogeny. They also suggest that TPO/c-Mpl signaling play a crucial role in the formation of hematopoietic cells in Xenopus.

Original languageEnglish
Pages (from-to)63-75
Number of pages13
JournalDevelopment Growth and Differentiation
Volume44
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Thrombopoietin
Hematopoiesis
Xenopus laevis
Mesoderm
Hematopoietic Stem Cells
Xenopus
Embryonic Structures
Bone Morphogenetic Protein 4
Blastula
Activins
Ectoderm
Erythrocyte Count
Leukocyte Count
Blood Cells
Intercellular Signaling Peptides and Proteins
Antibodies

Keywords

  • Activin
  • BMP-4
  • c-Mpl
  • Hematopoiesis
  • Thrombopoietin
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Role of the thrombopoietin (TPO)/Mpl system : c-Mpl-like molecule/TPO signaling enhances early hematopoiesis in Xenopus laevis. / Kakeda, Minoru; Kyuno, Jun Ichi; Kato, Takashi; Nishikawa, Mitsuo; Asashima, Makoto.

In: Development Growth and Differentiation, Vol. 44, No. 1, 2002, p. 63-75.

Research output: Contribution to journalArticle

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