Roles of type I Myosins in Drosophila handedness

Kiichiro Taniguchi, Shunya Hozumi, Reo Maeda, Takashi Okumura, Kenji Matsuno

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although bilateral animals, including Drosophila, appear to have left-right (LR) symmetry from the outside, their internal organs often show directional and stereotypical LR asymmetry. The mechanisms by which the LR axis is established in Drosophila have not been studied well. We showed that two type I Myosin proteins play crucial roles in the manifestation of Drosophila handedness. Mutants of Myosin31DF (Myo31DF), which encodes a type ID Myosin, showed reversed laterality of the embryonic and adult gut and testis. Myo31DF was required in the epithelial cells of the embryonic hindgut, where its protein co-localized with actin filaments, for the correct handedness of this organ. Disorganization of the actin cytoskeleton in the hindgut epithelium caused LR defects of the embryonic hindgut. These results suggest that the actin-based Myo31DF function is required for proper handedness. In contrast, the disruption of microtubules in the hindgut epithelium did not affect the laterality of this organ. We also found that the overexpression of Myosin61F (Myo61F), which encodes another type I Myosin in the hindgut epithelium reversed the hindgut handedness, suggesting that these two type I Myosins- Myo31DF and Myo61F-have antagonistic functions. We propose that the actin-based functions of type I Myosins play critical roles in generating LR asymmetry in invertebrates.

Original languageEnglish
JournalFly
Volume1
Issue number5
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

hindgut
myosin
Drosophila
epithelium
microfilaments
actin
microtubules
testes
epithelial cells
proteins
digestive system
invertebrates
mutants
animals

Keywords

  • Actin cytoskeleton
  • Foregut
  • Hindgut
  • Left-right asymmetry
  • Midgut
  • Myosin31DF
  • Myosin61F
  • Spermiduct
  • Type I MYOSIN family

ASJC Scopus subject areas

  • Insect Science

Cite this

Taniguchi, K., Hozumi, S., Maeda, R., Okumura, T., & Matsuno, K. (2007). Roles of type I Myosins in Drosophila handedness. Fly, 1(5).

Roles of type I Myosins in Drosophila handedness. / Taniguchi, Kiichiro; Hozumi, Shunya; Maeda, Reo; Okumura, Takashi; Matsuno, Kenji.

In: Fly, Vol. 1, No. 5, 2007.

Research output: Contribution to journalArticle

Taniguchi, K, Hozumi, S, Maeda, R, Okumura, T & Matsuno, K 2007, 'Roles of type I Myosins in Drosophila handedness', Fly, vol. 1, no. 5.
Taniguchi K, Hozumi S, Maeda R, Okumura T, Matsuno K. Roles of type I Myosins in Drosophila handedness. Fly. 2007;1(5).
Taniguchi, Kiichiro ; Hozumi, Shunya ; Maeda, Reo ; Okumura, Takashi ; Matsuno, Kenji. / Roles of type I Myosins in Drosophila handedness. In: Fly. 2007 ; Vol. 1, No. 5.
@article{d3279578cc27447cb8adceb833ed718a,
title = "Roles of type I Myosins in Drosophila handedness",
abstract = "Although bilateral animals, including Drosophila, appear to have left-right (LR) symmetry from the outside, their internal organs often show directional and stereotypical LR asymmetry. The mechanisms by which the LR axis is established in Drosophila have not been studied well. We showed that two type I Myosin proteins play crucial roles in the manifestation of Drosophila handedness. Mutants of Myosin31DF (Myo31DF), which encodes a type ID Myosin, showed reversed laterality of the embryonic and adult gut and testis. Myo31DF was required in the epithelial cells of the embryonic hindgut, where its protein co-localized with actin filaments, for the correct handedness of this organ. Disorganization of the actin cytoskeleton in the hindgut epithelium caused LR defects of the embryonic hindgut. These results suggest that the actin-based Myo31DF function is required for proper handedness. In contrast, the disruption of microtubules in the hindgut epithelium did not affect the laterality of this organ. We also found that the overexpression of Myosin61F (Myo61F), which encodes another type I Myosin in the hindgut epithelium reversed the hindgut handedness, suggesting that these two type I Myosins- Myo31DF and Myo61F-have antagonistic functions. We propose that the actin-based functions of type I Myosins play critical roles in generating LR asymmetry in invertebrates.",
keywords = "Actin cytoskeleton, Foregut, Hindgut, Left-right asymmetry, Midgut, Myosin31DF, Myosin61F, Spermiduct, Type I MYOSIN family",
author = "Kiichiro Taniguchi and Shunya Hozumi and Reo Maeda and Takashi Okumura and Kenji Matsuno",
year = "2007",
language = "English",
volume = "1",
journal = "Fly",
issn = "1933-6934",
publisher = "Landes Bioscience",
number = "5",

}

TY - JOUR

T1 - Roles of type I Myosins in Drosophila handedness

AU - Taniguchi, Kiichiro

AU - Hozumi, Shunya

AU - Maeda, Reo

AU - Okumura, Takashi

AU - Matsuno, Kenji

PY - 2007

Y1 - 2007

N2 - Although bilateral animals, including Drosophila, appear to have left-right (LR) symmetry from the outside, their internal organs often show directional and stereotypical LR asymmetry. The mechanisms by which the LR axis is established in Drosophila have not been studied well. We showed that two type I Myosin proteins play crucial roles in the manifestation of Drosophila handedness. Mutants of Myosin31DF (Myo31DF), which encodes a type ID Myosin, showed reversed laterality of the embryonic and adult gut and testis. Myo31DF was required in the epithelial cells of the embryonic hindgut, where its protein co-localized with actin filaments, for the correct handedness of this organ. Disorganization of the actin cytoskeleton in the hindgut epithelium caused LR defects of the embryonic hindgut. These results suggest that the actin-based Myo31DF function is required for proper handedness. In contrast, the disruption of microtubules in the hindgut epithelium did not affect the laterality of this organ. We also found that the overexpression of Myosin61F (Myo61F), which encodes another type I Myosin in the hindgut epithelium reversed the hindgut handedness, suggesting that these two type I Myosins- Myo31DF and Myo61F-have antagonistic functions. We propose that the actin-based functions of type I Myosins play critical roles in generating LR asymmetry in invertebrates.

AB - Although bilateral animals, including Drosophila, appear to have left-right (LR) symmetry from the outside, their internal organs often show directional and stereotypical LR asymmetry. The mechanisms by which the LR axis is established in Drosophila have not been studied well. We showed that two type I Myosin proteins play crucial roles in the manifestation of Drosophila handedness. Mutants of Myosin31DF (Myo31DF), which encodes a type ID Myosin, showed reversed laterality of the embryonic and adult gut and testis. Myo31DF was required in the epithelial cells of the embryonic hindgut, where its protein co-localized with actin filaments, for the correct handedness of this organ. Disorganization of the actin cytoskeleton in the hindgut epithelium caused LR defects of the embryonic hindgut. These results suggest that the actin-based Myo31DF function is required for proper handedness. In contrast, the disruption of microtubules in the hindgut epithelium did not affect the laterality of this organ. We also found that the overexpression of Myosin61F (Myo61F), which encodes another type I Myosin in the hindgut epithelium reversed the hindgut handedness, suggesting that these two type I Myosins- Myo31DF and Myo61F-have antagonistic functions. We propose that the actin-based functions of type I Myosins play critical roles in generating LR asymmetry in invertebrates.

KW - Actin cytoskeleton

KW - Foregut

KW - Hindgut

KW - Left-right asymmetry

KW - Midgut

KW - Myosin31DF

KW - Myosin61F

KW - Spermiduct

KW - Type I MYOSIN family

UR - http://www.scopus.com/inward/record.url?scp=84883356140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883356140&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84883356140

VL - 1

JO - Fly

JF - Fly

SN - 1933-6934

IS - 5

ER -