TY - JOUR
T1 - Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions
AU - Takeoka, Shinji
AU - Teramura, Yuji
AU - Okamura, Yosuke
AU - Tsuchida, Eishun
AU - Handa, Makoto
AU - Ikeda, Yasuo
PY - 2002/10/16
Y1 - 2002/10/16
N2 - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
AB - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
KW - Artificial platelets
KW - Fluorescent anisotropy
KW - GPIbα
KW - Glycoprotein
KW - Membrane flexibility
KW - Phospholipid
KW - Platelets
KW - Rolling
KW - Vesicles
KW - Von Willebrand factor
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U2 - 10.1016/S0006-291X(02)00934-8
DO - 10.1016/S0006-291X(02)00934-8
M3 - Article
C2 - 12176049
AN - SCOPUS:0035984323
VL - 296
SP - 765
EP - 770
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -