Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions

Shinji Takeoka, Yuji Teramura, Yosuke Okamura, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

    Original languageEnglish
    Pages (from-to)765-770
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume296
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    von Willebrand Factor
    Phospholipids
    Diphenylhexatriene
    Artificial Membranes
    Platelet Membrane Glycoproteins
    Membranes
    Anisotropy
    Blood Platelets
    Formability
    Platelets
    N-succinimidyl 3-(2-pyridyldithio)propionate

    Keywords

    • Artificial platelets
    • Fluorescent anisotropy
    • Glycoprotein
    • GPIbα
    • Membrane flexibility
    • Phospholipid
    • Platelets
    • Rolling
    • Vesicles
    • Von Willebrand factor

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions. / Takeoka, Shinji; Teramura, Yuji; Okamura, Yosuke; Tsuchida, Eishun; Handa, Makoto; Ikeda, Yasuo.

    In: Biochemical and Biophysical Research Communications, Vol. 296, No. 3, 2002, p. 765-770.

    Research output: Contribution to journalArticle

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    AU - Tsuchida, Eishun

    AU - Handa, Makoto

    AU - Ikeda, Yasuo

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