Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions

Shinji Takeoka, Yuji Teramura, Yosuke Okamura, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

Original languageEnglish
Pages (from-to)765-770
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number3
DOIs
Publication statusPublished - 2002 Oct 16

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Keywords

  • Artificial platelets
  • Fluorescent anisotropy
  • GPIbα
  • Glycoprotein
  • Membrane flexibility
  • Phospholipid
  • Platelets
  • Rolling
  • Vesicles
  • Von Willebrand factor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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