Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions

Shinji Takeoka, Yuji Teramura, Yosuke Okamura, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

    Research output: Contribution to journal › Article

    33 Citations (Scopus)

    Abstract

    The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

    Original languageEnglish
    Pages (from-to)765-770
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume296
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    von Willebrand Factor
    Phospholipids
    Diphenylhexatriene
    Artificial Membranes
    Platelet Membrane Glycoproteins
    Membranes
    Anisotropy
    Blood Platelets
    Formability
    Platelets
    N-succinimidyl 3-(2-pyridyldithio)propionate

    Keywords

    • Artificial platelets
    • Fluorescent anisotropy
    • Glycoprotein
    • GPIbα
    • Membrane flexibility
    • Phospholipid
    • Platelets
    • Rolling
    • Vesicles
    • Von Willebrand factor

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions. / Takeoka, Shinji; Teramura, Yuji; Okamura, Yosuke; Tsuchida, Eishun; Handa, Makoto; Ikeda, Yasuo.

    In: Biochemical and Biophysical Research Communications, Vol. 296, No. 3, 2002, p. 765-770.

    Research output: Contribution to journal › Article

    Takeoka, S, Teramura, Y, Okamura, Y, Tsuchida, E, Handa, M & Ikeda, Y 2002, 'Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions', Biochemical and Biophysical Research Communications, vol. 296, no. 3, pp. 765-770. https://doi.org/10.1016/S0006-291X(02)00934-8
    Takeoka S, Teramura Y, Okamura Y, Tsuchida E, Handa M, Ikeda Y. Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions. Biochemical and Biophysical Research Communications. 2002;296(3):765-770. https://doi.org/10.1016/S0006-291X(02)00934-8
    Takeoka, Shinji ; Teramura, Yuji ; Okamura, Yosuke ; Tsuchida, Eishun ; Handa, Makoto ; Ikeda, Yasuo. / Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 296, No. 3. pp. 765-770.
    @article{1670bf5a67b04026bc8fb27b771514bc,
    title = "Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions",
    abstract = "The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.",
    keywords = "Artificial platelets, Fluorescent anisotropy, Glycoprotein, GPIbα, Membrane flexibility, Phospholipid, Platelets, Rolling, Vesicles, Von Willebrand factor",
    author = "Shinji Takeoka and Yuji Teramura and Yosuke Okamura and Eishun Tsuchida and Makoto Handa and Yasuo Ikeda",
    year = "2002",
    doi = "10.1016/S0006-291X(02)00934-8",
    language = "English",
    volume = "296",
    pages = "765--770",
    journal = "Biochemical and Biophysical Research Communications",
    issn = "0006-291X",
    publisher = "Academic Press Inc.",
    number = "3",

    }

    TY - JOUR

    T1 - Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions

    AU - Takeoka, Shinji

    AU - Teramura, Yuji

    AU - Okamura, Yosuke

    AU - Tsuchida, Eishun

    AU - Handa, Makoto

    AU - Ikeda, Yasuo

    PY - 2002

    Y1 - 2002

    N2 - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

    AB - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

    KW - Artificial platelets

    KW - Fluorescent anisotropy

    KW - Glycoprotein

    KW - GPIbα

    KW - Membrane flexibility

    KW - Phospholipid

    KW - Platelets

    KW - Rolling

    KW - Vesicles

    KW - Von Willebrand factor

    UR - http://www.scopus.com/inward/record.url?scp=0035984323&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0035984323&partnerID=8YFLogxK

    U2 - 10.1016/S0006-291X(02)00934-8

    DO - 10.1016/S0006-291X(02)00934-8

    M3 - Article

    VL - 296

    SP - 765

    EP - 770

    JO - Biochemical and Biophysical Research Communications

    JF - Biochemical and Biophysical Research Communications

    SN - 0006-291X

    IS - 3

    ER -

    Access to Document