Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis

T. Hirose, Y. Hoshino, D. H. Yoon, T. Mori, T. Sekiguchi, H. Takeyama, S. Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 %. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 %.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages440-442
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Cell encapsulation
  • Gel micro droplet (GMD)
  • Microfluidics
  • Single cell

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Hirose, T., Hoshino, Y., Yoon, D. H., Mori, T., Sekiguchi, T., Takeyama, H., & Shoji, S. (2013). Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 440-442). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.