Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 %. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 %.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages440-442
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Sepharose
Gels
Throughput
Temperature
Microfluidics
Temperature control
Escherichia coli
Capsules
Liquids

Keywords

  • Cell encapsulation
  • Gel micro droplet (GMD)
  • Microfluidics
  • Single cell

ASJC Scopus subject areas

  • Bioengineering

Cite this

Hirose, T., Hoshino, Y., Yoon, D., Mori, T., Sekiguchi, T., Takeyama, H., & Shoji, S. (2013). Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 440-442). Chemical and Biological Microsystems Society.

Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. / Hirose, T.; Hoshino, Y.; Yoon, Donghyun; Mori, T.; Sekiguchi, Tetsushi; Takeyama, Haruko; Shoji, Shuichi.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 440-442.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hirose, T, Hoshino, Y, Yoon, D, Mori, T, Sekiguchi, T, Takeyama, H & Shoji, S 2013, Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 440-442, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, 13/10/27.
Hirose T, Hoshino Y, Yoon D, Mori T, Sekiguchi T, Takeyama H et al. Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 440-442
Hirose, T. ; Hoshino, Y. ; Yoon, Donghyun ; Mori, T. ; Sekiguchi, Tetsushi ; Takeyama, Haruko ; Shoji, Shuichi. / Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 440-442
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