Rotational catalysis by F1-ATPase

K. Adachi, T. Nishizaka, K. Kinosita

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

F1-ATPase, a water-soluble portion of ATP synthase, is a fully reversible rotary molecular machine in which a central γ subunit rotates inside a cylinder made of three α and three β subunits alternately arranged. This motor rotates counterclockwise by hydrolyzing ATP in three catalytic sites but synthesizes ATP when forced to rotate clockwise by an external force. Single-molecule studies have revealed how the chemical reactions that occur in the three catalytic sites are coupled to mechanical rotation.

Original languageEnglish
Title of host publicationComprehensive Biophysics
PublisherElsevier Inc.
Pages35-49
Number of pages15
Volume8
ISBN (Print)9780080957180
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Proton-Translocating ATPases
Catalysis
Adenosine Triphosphate
Catalytic Domain
Chemical reactions
Molecules
Water

Keywords

  • ATP synthase
  • Chemo-mechanical coupling
  • Conformational change
  • F-ATPase
  • Fluorescent ATP analog
  • Free energy
  • High efficiency
  • High-speed imaging
  • Induced fit
  • Molecular motor
  • Power stroke
  • Single fluorophore imaging
  • Single-molecule study
  • Torque generation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Adachi, K., Nishizaka, T., & Kinosita, K. (2012). Rotational catalysis by F1-ATPase. In Comprehensive Biophysics (Vol. 8, pp. 35-49). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-374920-8.00804-3

Rotational catalysis by F1-ATPase. / Adachi, K.; Nishizaka, T.; Kinosita, K.

Comprehensive Biophysics. Vol. 8 Elsevier Inc., 2012. p. 35-49.

Research output: Chapter in Book/Report/Conference proceedingChapter

Adachi, K, Nishizaka, T & Kinosita, K 2012, Rotational catalysis by F1-ATPase. in Comprehensive Biophysics. vol. 8, Elsevier Inc., pp. 35-49. https://doi.org/10.1016/B978-0-12-374920-8.00804-3
Adachi K, Nishizaka T, Kinosita K. Rotational catalysis by F1-ATPase. In Comprehensive Biophysics. Vol. 8. Elsevier Inc. 2012. p. 35-49 https://doi.org/10.1016/B978-0-12-374920-8.00804-3
Adachi, K. ; Nishizaka, T. ; Kinosita, K. / Rotational catalysis by F1-ATPase. Comprehensive Biophysics. Vol. 8 Elsevier Inc., 2012. pp. 35-49
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