Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface

Hiroshi Osada, Mamoru Nakanishi, Masamichi Tsuboi, Kazuhiko Kinosita, Akira Ikegami

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.

Original languageEnglish
Pages (from-to)321-324
Number of pages4
JournalBBA - Biomembranes
Volume773
Issue number2
DOIs
Publication statusPublished - 1984 Jun 27
Externally publishedYes

Fingerprint

Haptens
Immunoglobulins
Membranes
Membrane Fluidity
Spectrum Analysis
Fluidity
Immunoglobulin G
Fluorescence
Rabbits
Lipids
Antibodies
Spectroscopy

Keywords

  • (Liposome)
  • Fluorescence anisotropy
  • Immunoglobulin
  • Lipid hapten
  • Membrane fluidity
  • Rotational dynamics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface. / Osada, Hiroshi; Nakanishi, Mamoru; Tsuboi, Masamichi; Kinosita, Kazuhiko; Ikegami, Akira.

In: BBA - Biomembranes, Vol. 773, No. 2, 27.06.1984, p. 321-324.

Research output: Contribution to journalArticle

Osada, H, Nakanishi, M, Tsuboi, M, Kinosita, K & Ikegami, A 1984, 'Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface', BBA - Biomembranes, vol. 773, no. 2, pp. 321-324. https://doi.org/10.1016/0005-2736(84)90097-X
Osada, Hiroshi ; Nakanishi, Mamoru ; Tsuboi, Masamichi ; Kinosita, Kazuhiko ; Ikegami, Akira. / Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface. In: BBA - Biomembranes. 1984 ; Vol. 773, No. 2. pp. 321-324.
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