RpaB, another response regulator operating circadian clock-dependent transcriptional regulation in Synechococcus elongatus PCC 7942

Mitsumasa Hanaoka, Naoki Takai, Norimune Hosokawa, Masayuki Fujiwara, Yuki Akimoto, Nami Kobori, Hideo Iwasaki, Takao Kondo, Kan Tanaka

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    The circadian clock of cyanobacteria is composed of KaiA, KaiB, and KaiC proteins, and the SasA-RpaA two-component system has been implicated in the regulation of one of the output pathways of the clock. In this study, we show that another response regulator that is essential for viability, the RpaA paralog, RpaB, plays a central role in the transcriptional oscillation of clock-regulated genes. In vivo and in vitro analyses revealed that RpaB and not RpaA could specifically bind to the kaiBC promoter, possibly repressing transcription during subjective night. This suggested that binding may be terminated by RpaA to activate gene transcription during subjective day. Moreover, we found that rpoD6 and sigF2, which encode group-2 and group-3 σ factors for RNA polymerase, respectively, were also targets of the RpaAB system, suggesting that a specific group of σ factors can propagate genome-wide transcriptional oscillation. Our findings thus reveal a novel mechanism for a circadian output pathway that is mediated by two paralogous response regulators.

    Original languageEnglish
    Pages (from-to)26321-26327
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume287
    Issue number31
    DOIs
    Publication statusPublished - 2012 Jul 27

    Fingerprint

    Synechococcus
    Circadian Clocks
    Clocks
    Genes
    Transcription
    Cyanobacteria
    DNA-Directed RNA Polymerases
    Genome
    Proteins
    In Vitro Techniques

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Cite this

    RpaB, another response regulator operating circadian clock-dependent transcriptional regulation in Synechococcus elongatus PCC 7942. / Hanaoka, Mitsumasa; Takai, Naoki; Hosokawa, Norimune; Fujiwara, Masayuki; Akimoto, Yuki; Kobori, Nami; Iwasaki, Hideo; Kondo, Takao; Tanaka, Kan.

    In: Journal of Biological Chemistry, Vol. 287, No. 31, 27.07.2012, p. 26321-26327.

    Research output: Contribution to journalArticle

    Hanaoka, Mitsumasa ; Takai, Naoki ; Hosokawa, Norimune ; Fujiwara, Masayuki ; Akimoto, Yuki ; Kobori, Nami ; Iwasaki, Hideo ; Kondo, Takao ; Tanaka, Kan. / RpaB, another response regulator operating circadian clock-dependent transcriptional regulation in Synechococcus elongatus PCC 7942. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 31. pp. 26321-26327.
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    abstract = "The circadian clock of cyanobacteria is composed of KaiA, KaiB, and KaiC proteins, and the SasA-RpaA two-component system has been implicated in the regulation of one of the output pathways of the clock. In this study, we show that another response regulator that is essential for viability, the RpaA paralog, RpaB, plays a central role in the transcriptional oscillation of clock-regulated genes. In vivo and in vitro analyses revealed that RpaB and not RpaA could specifically bind to the kaiBC promoter, possibly repressing transcription during subjective night. This suggested that binding may be terminated by RpaA to activate gene transcription during subjective day. Moreover, we found that rpoD6 and sigF2, which encode group-2 and group-3 σ factors for RNA polymerase, respectively, were also targets of the RpaAB system, suggesting that a specific group of σ factors can propagate genome-wide transcriptional oscillation. Our findings thus reveal a novel mechanism for a circadian output pathway that is mediated by two paralogous response regulators.",
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    AU - Hanaoka, Mitsumasa

    AU - Takai, Naoki

    AU - Hosokawa, Norimune

    AU - Fujiwara, Masayuki

    AU - Akimoto, Yuki

    AU - Kobori, Nami

    AU - Iwasaki, Hideo

    AU - Kondo, Takao

    AU - Tanaka, Kan

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