Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta

Watsapon Wattanasuepsin, Bungonsiri Intra, Akira Take, Yuki Inahashi, Jirayut Euanorasetr, Satoshi Ōmura, Atsuko Matsumoto, Watanalai Panbangred

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Abstract

A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).

Original languageEnglish
Pages (from-to)4572-4577
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1
Externally publishedYes

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Keywords

  • Rhizosphere
  • Saccharomonospora

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Wattanasuepsin, W., Intra, B., Take, A., Inahashi, Y., Euanorasetr, J., Ōmura, S., Matsumoto, A., & Panbangred, W. (2017). Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. International Journal of Systematic and Evolutionary Microbiology, 67(11), 4572-4577. https://doi.org/10.1099/ijsem.0.002336