Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta

Watsapon Wattanasuepsin, Bungonsiri Intra, Akira Take, Yuki Inahashi, Jirayut Euanorasetr, Satoshi Ōmura, Atsuko Matsumoto, Watanalai Panbangred

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).

Original languageEnglish
Pages (from-to)4572-4577
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1
Externally publishedYes

Fingerprint

Saccharomonospora
Colocasia
Mycolic Acids
Diaminopimelic Acid
Vitamin K 2
Colocasia esculenta
Rhizosphere
Arabinose
Cardiolipins
Hyphae
Actinobacteria
Mycelium
Base Composition
Thailand
Spores
Galactose
rRNA Genes
Cell Wall
Names
Ear

Keywords

  • Rhizosphere
  • Saccharomonospora

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. / Wattanasuepsin, Watsapon; Intra, Bungonsiri; Take, Akira; Inahashi, Yuki; Euanorasetr, Jirayut; Ōmura, Satoshi; Matsumoto, Atsuko; Panbangred, Watanalai.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 11, 01.11.2017, p. 4572-4577.

Research output: Contribution to journalArticle

Wattanasuepsin, W, Intra, B, Take, A, Inahashi, Y, Euanorasetr, J, Ōmura, S, Matsumoto, A & Panbangred, W 2017, 'Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 11, pp. 4572-4577. https://doi.org/10.1099/ijsem.0.002336
Wattanasuepsin, Watsapon ; Intra, Bungonsiri ; Take, Akira ; Inahashi, Yuki ; Euanorasetr, Jirayut ; Ōmura, Satoshi ; Matsumoto, Atsuko ; Panbangred, Watanalai. / Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. In: International Journal of Systematic and Evolutionary Microbiology. 2017 ; Vol. 67, No. 11. pp. 4572-4577.
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abstract = "A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol{\%}. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1{\%}) and Saccharomonospora viridis DSM 43017T (97.1{\%}) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6{\%}). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50{\%}, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).",
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