Safe use of radioisotopes.

Jill Meisenhelder, Kentaro Senba

Research output: Contribution to journalArticle

Abstract

The use of radioisotopes to label specific molecules in a defined way has greatly furthered the discovery and dissection of biochemical pathways. The development of methods to synthesize such tagged biological compounds inexpensively on an industrial scale has enabled them to be used routinely in laboratory protocols, including many detailed in this manual. Although most of these protocols involve the use of only microcurie amounts of radioactivity, some (particularly those describing the metabolic labeling of proteins or nucleic acids within cells) require amounts on the order of millicuries. In all cases where radioisotopes are used, depending on the quantity and nature of the isotope, certain precautions must be taken to ensure the safety of the scientist. It is essential to use good safety practices and proper protection to handle radioactive substances. This unit discusses handling, storage, and disposal of the isotopes most frequently used in biological research.

Original languageEnglish
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeAppendix 1
Publication statusPublished - 2006 Sep
Externally publishedYes

Fingerprint

Radioisotopes
Isotopes
Safety
Radioactivity
Nucleic Acids
Dissection
Research
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Safe use of radioisotopes. / Meisenhelder, Jill; Senba, Kentaro.

In: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Appendix 1, 09.2006.

Research output: Contribution to journalArticle

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