Salinity-dependent copy number increase of a marine cyanobacterial endogenous plasmid

Haruko Takeyama; J. Grant Burgess; Hiroaki Sudo; Koji Sode; Tadashi Matsunaga

doi:10.1016/0378-1097(91)90653-R

Salinity-dependent copy number increase of a marine cyanobacterial endogenous plasmid

Haruko Takeyama, J. Grant Burgess, Hiroaki Sudo, Koji Sode, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A marine cyanobacterium Synechococcus sp. NKBG 042902 grown in the presence of various NaCl concentrations showed different plasmid profiles. This strain possesses a 2.7-kb endogenous plasmid pSY10, whose copy number increased five-fold when the salinity of the growth medium was increased from 0 to 3% NaCl. Cells were grown initially in a fresh water medium and then transferred to a NaCl supplemented medium. The copy number of pSY10 began to increase after 7 h of incubation in 3% NaCl BG11 medium. This phenomenon was specific to NaCl and did not occur when other osmotica such as KCl and sorbitol were used. This is the first report of a cyanobacterial plasmid with a salinity-dependent copy number.

Original language	English
Pages (from-to)	95-98
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(91)90653-R
Publication status	Published - 1991 Dec 15
Externally published	Yes

Keywords

Copy number
Marine cyanobacterium
Plasmid
Sodium chloride
Synechococcus

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/0378-1097(91)90653-R

Cite this

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title = "Salinity-dependent copy number increase of a marine cyanobacterial endogenous plasmid",

abstract = "A marine cyanobacterium Synechococcus sp. NKBG 042902 grown in the presence of various NaCl concentrations showed different plasmid profiles. This strain possesses a 2.7-kb endogenous plasmid pSY10, whose copy number increased five-fold when the salinity of the growth medium was increased from 0 to 3% NaCl. Cells were grown initially in a fresh water medium and then transferred to a NaCl supplemented medium. The copy number of pSY10 began to increase after 7 h of incubation in 3% NaCl BG11 medium. This phenomenon was specific to NaCl and did not occur when other osmotica such as KCl and sorbitol were used. This is the first report of a cyanobacterial plasmid with a salinity-dependent copy number.",

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AU - Takeyama, Haruko

AU - Grant Burgess, J.

AU - Sudo, Hiroaki

AU - Sode, Koji

AU - Matsunaga, Tadashi

PY - 1991/12/15

Y1 - 1991/12/15

N2 - A marine cyanobacterium Synechococcus sp. NKBG 042902 grown in the presence of various NaCl concentrations showed different plasmid profiles. This strain possesses a 2.7-kb endogenous plasmid pSY10, whose copy number increased five-fold when the salinity of the growth medium was increased from 0 to 3% NaCl. Cells were grown initially in a fresh water medium and then transferred to a NaCl supplemented medium. The copy number of pSY10 began to increase after 7 h of incubation in 3% NaCl BG11 medium. This phenomenon was specific to NaCl and did not occur when other osmotica such as KCl and sorbitol were used. This is the first report of a cyanobacterial plasmid with a salinity-dependent copy number.

AB - A marine cyanobacterium Synechococcus sp. NKBG 042902 grown in the presence of various NaCl concentrations showed different plasmid profiles. This strain possesses a 2.7-kb endogenous plasmid pSY10, whose copy number increased five-fold when the salinity of the growth medium was increased from 0 to 3% NaCl. Cells were grown initially in a fresh water medium and then transferred to a NaCl supplemented medium. The copy number of pSY10 began to increase after 7 h of incubation in 3% NaCl BG11 medium. This phenomenon was specific to NaCl and did not occur when other osmotica such as KCl and sorbitol were used. This is the first report of a cyanobacterial plasmid with a salinity-dependent copy number.

KW - Copy number

KW - Marine cyanobacterium

KW - Plasmid

KW - Sodium chloride

KW - Synechococcus

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Salinity-dependent copy number increase of a marine cyanobacterial endogenous plasmid

Abstract

Keywords

ASJC Scopus subject areas

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