Salinity-regulated replication of the endogenous plasmid pSY10 from the marine cyanobacterium Synechococcus sp.

Haruko Takeyama, Hideki Nakayama, Tadashi Matsunaga

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The endogenous plasmid pSY10 in the marine cyanobacterium Synechococcus sp. NKBG042902 is maintained at a high copy number when cells are grown in seawater and at a low copy number when cultured in freshwater. The mechanism of salinity-regulated replication of this plasmid was investigated. Transcription of repA was depressed under freshwater, which was accompanied by a low copy number of pSY10 and the appearance of a new protein that was expressed only in cells cultured in freshwater. This protein was observed to bind to putative repA promoters (Prep1 and Prep2) on pSY10. Moreover, this protein was observed only in Synechococcus sp. NKBG042902. The data suggest that this protein(s) regulates repA transcription in pSY10, stress responsive and encoded by the host chromosome.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume84-86
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Synechococcus
Salinity
Cyanobacteria
Plasmids
Fresh Water
Proteins
Transcription
Seawater
Chromosomes
Cultured Cells
Cell Count

Keywords

  • Band shift assay
  • Copy number
  • Marine cyanobacterium
  • Plasmid pSY10
  • repA gene
  • Replication
  • Salinity stress
  • Synechococcus sp.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

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abstract = "The endogenous plasmid pSY10 in the marine cyanobacterium Synechococcus sp. NKBG042902 is maintained at a high copy number when cells are grown in seawater and at a low copy number when cultured in freshwater. The mechanism of salinity-regulated replication of this plasmid was investigated. Transcription of repA was depressed under freshwater, which was accompanied by a low copy number of pSY10 and the appearance of a new protein that was expressed only in cells cultured in freshwater. This protein was observed to bind to putative repA promoters (Prep1 and Prep2) on pSY10. Moreover, this protein was observed only in Synechococcus sp. NKBG042902. The data suggest that this protein(s) regulates repA transcription in pSY10, stress responsive and encoded by the host chromosome.",
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AU - Takeyama, Haruko

AU - Nakayama, Hideki

AU - Matsunaga, Tadashi

PY - 2000

Y1 - 2000

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AB - The endogenous plasmid pSY10 in the marine cyanobacterium Synechococcus sp. NKBG042902 is maintained at a high copy number when cells are grown in seawater and at a low copy number when cultured in freshwater. The mechanism of salinity-regulated replication of this plasmid was investigated. Transcription of repA was depressed under freshwater, which was accompanied by a low copy number of pSY10 and the appearance of a new protein that was expressed only in cells cultured in freshwater. This protein was observed to bind to putative repA promoters (Prep1 and Prep2) on pSY10. Moreover, this protein was observed only in Synechococcus sp. NKBG042902. The data suggest that this protein(s) regulates repA transcription in pSY10, stress responsive and encoded by the host chromosome.

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